Recent Advances in Biotechnology

Volume: 5

Sperm Assessment and Processing

Author(s): Krishnavignesh Lakshmanan, Deepa Onnarakatt*, V. M. Thomas, Smisha Sridev, Anuja Gopalakrishnan and Poongothai Muthuswamy

Pp: 60-89 (30)

DOI: 10.2174/9789815051667122050004

* (Excluding Mailing and Handling)


Semen analysis has become a definitive tool for understanding the male fertility factor. The volume of the ejaculation indicates the functioning and patency of testicular organs, whereas the quantity and quality of sperm define one’s fertility profile. Thus, semen analysis is the cornerstone for diagnosis as well as for medical management. Semen comes with both cellular and non-cellular components. For the management of male infertility, it is necessary to have better quality sperm. As a result, semen processing has become an integral part of any medically assisted reproduction. There are different standardized preparation methods available that include sperm wash, swim-up technique, pelleting method, density gradient, etc. The aim of this chapter is to discuss semen analysis, its relevance in understanding male fertility factors, and the available sperm preparation methods. Based on the literature, it can be concluded that a proper semen assessment, according to the latest WHO guidelines, is mandatory for diagnosing male fertility issues. The appropriate sperm preparation method and good quality of sperms combined with the skill set of embryologists and environmental conditions will be the decisive factor in any IUI/IVF/ART success.

Keywords: Density gradient, Pelleting method, Semen analysis, Semen processing, Sperm wash, Swim-up technique.

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