The recent emergence of the novel betacoronavirus, pathogenic severe acute
respiratory syndrome-coronavirus-2 (SARS-CoV-2) with high nucleotide identity to
SARS-CoV represents the causative agent of a potentially deadly disease coronavirus
disease-19 (COVID-19) in Wuhan, China, and spreading across several countries
globally pose a great global public health concern. Until a vaccine is available,
effective therapy must be identified, and many clinical trialshave been executed
worldwide. Various in vitro investigations are ongoing using different cell cultures to
find alternative treatment options, allowing SARS-CoV-2 replications. Various cell
lines are susceptible to the SARS-CoV-2 infection. In this chapter, literature regarding
SARS-CoV-2 isolated in several cell lines commonly used for diagnostic or research
purposes has been summarized. It also shows that SARS-CoV-2 can achieve high titers
in various cell cultures derived from different species. In addition, these cell lines are
extensively used in the diagnosis, to study pathophysiology, genome studies, and the
finding of new targets for drug development and provide new ideas for the discovery of
lead compounds with potential therapeutic agents against novel COVID-19.
Keywords: Anti-viral, Betacoronavirus, Cell lines, Clinical trials, Lead
compounds, SARS-CoV-2.
