Abiotic stresses have contributed to the generation of reactive oxygen
species called as free radicals which are highly toxic to the organism. Free radicals may
be evaluated either explicitly or inadvertently after the production of oxidative byproducts
of nucleic acids, proteins or lipids, a method also known as fingerprinting.
Though the approaches for analyzing such reactive intermediates have been thoroughly
studied; we concentrated primarily on recent implementations of these techniques to
quantify free radicals and different candidate biomarkers of oxidative stress such as
nitrotyrosine, isoprostane, etc. Further, the various biochemical approaches along with
the conventional methods are also discussed for the evaluation of antioxidant activity
of natural products.
Keywords: Antioxidants, Biochemical approaches, Biomarkers, Oxidative stress.
