The World Health Organization (WHO) has indicated coronavirus disease-
19 (COVID-19) as a global pandemic. A virus that is formed by the recombination
process and whose genetic material as well as protein-rich envelope are resulting out of
various viruses is known as pseudovirus. There are certain advantages of using
pseudovirus over native viruses. Due to several novel viruses e.g., Marburg virus,
Ebola virus, Lassa fever virus, immunodeficiency virus, and coronavirus, there is a
development of numerous packing arrangements to produce pseudotype virus having
envelope in their structure. This chapter deals with various virus packaging systems
with a focus on production and neutralization assay of pseudovirus.
Keywords: Assay, COVID-19, Pseudovirus, SARS.
