High Performance Liquid Chromatography (HPLC) is a powerful
chromatographic technique to separate, identify and quantify components in a mixture.
HPLC has superior features such as versatility, safety, sensitivity, accuracy and low
detection capability. It mainly utilizes a column that separates components, a pump that
delivers mobile phase through the column and a detector that detects the eluted
components from the column. Separation of components occurs based on the
interaction between the stationary phase and the components. HPLC is divided into
several classes depending on the stationary phase and the separation process such as
normal phase, reversed phase, and ion chromatography. It has significantly contributed
to many fields of science such as pharmaceuticals, foods, environment and forensics.
This chapter mainly focuses on the theoretical and practical aspects of HPLC.
Keywords: High performance liquid chromatography, HPLC analysis, HPLC
detector, Mobile phase, Normal phase chromatography, Reversed phase
chromatography, Stationary phase.
