The foundation knowledge of recent advancements of neuroscience was
based on the Golgi staining observations. This is one of the best approaches to visualise
the neuronal cytoarchitecture and complete morphology of neurons with incomparable
clarity. This technique is based on the principle of heavy metal impregnation. There are
many modifications and advancement occurred to improve the visualization. This
chapter will provide the recently used protocols to visuals the neuronal architecture,
dendritic arborization and spine density in different brain regions. Along with the
manual observation, the present chapter also describes the currently used tools and
software for the better understanding and visualisation of neurons.
Keywords: Brain, Dendritic branching, FIJI tool, Golgi staining, Metal, Neuronal
morphology, Neuron, Spine count, Sholl analysis, Software, Transcardial
perfusion.
