Abstract
A variety of multicolor fluorescence in situ hybridization (FISH) assays have been developed in the last decade. Routine application of such techniques started in 1996 with the simultaneous use of all the 24 human whole chromosome painting probes (i.e. multiplex-FISH = M-FISH and spectral karyotyping = SKY). Since that time different approaches for chromosomal differentiation based on multicolor-FISH (mFISH) assays have been published with the purpose to characterize structurally abnormal chromosomes and supernumerary marker chromosomes of unknown origin after conventional karyotypic analysis. Their characterization is of high clinical impact and is the requisite condition for further molecular investigations aimed at identification of disease related genes. We present an overview of the available different mFISH methods, highlighting their advantages and limitations, as well as their applications in clinical and tumor cytogenetics. Finally, an outlook is given on further possible developments of thi s special field of molecular cytogenetics.
Keywords: fish, m-fish, tumor cytogenetics
Current Genomics
Title: Multicolor-FISH Approaches for the Characterization of Human Chromosomes in Clinical Genetics and Tumor Cytogenetics
Volume: 3 Issue: 3
Author(s): Thomas Liehr and Uwe Claussen
Affiliation:
Keywords: fish, m-fish, tumor cytogenetics
Abstract: A variety of multicolor fluorescence in situ hybridization (FISH) assays have been developed in the last decade. Routine application of such techniques started in 1996 with the simultaneous use of all the 24 human whole chromosome painting probes (i.e. multiplex-FISH = M-FISH and spectral karyotyping = SKY). Since that time different approaches for chromosomal differentiation based on multicolor-FISH (mFISH) assays have been published with the purpose to characterize structurally abnormal chromosomes and supernumerary marker chromosomes of unknown origin after conventional karyotypic analysis. Their characterization is of high clinical impact and is the requisite condition for further molecular investigations aimed at identification of disease related genes. We present an overview of the available different mFISH methods, highlighting their advantages and limitations, as well as their applications in clinical and tumor cytogenetics. Finally, an outlook is given on further possible developments of thi s special field of molecular cytogenetics.
Export Options
About this article
Cite this article as:
Liehr Thomas and Claussen Uwe, Multicolor-FISH Approaches for the Characterization of Human Chromosomes in Clinical Genetics and Tumor Cytogenetics, Current Genomics 2002; 3 (3) . https://dx.doi.org/10.2174/1389202023350525
DOI https://dx.doi.org/10.2174/1389202023350525 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced AI Techniques in Big Genomic Data Analysis
The thematic issue on "Advanced AI Techniques in Big Genomic Data Analysis" aims to explore the cutting-edge methodologies and applications of artificial intelligence (AI) in the realm of genomic research, where vast amounts of data pose both challenges and opportunities. This issue will cover a broad spectrum of AI-driven strategies, ...read more
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Deep learning in Single Cell Analysis
The field of biology is undergoing a revolution in our ability to study individual cells at the molecular level, and to integrate data from multiple sources and modalities. This has been made possible by advances in technologies for single-cell sequencing, multi-omics profiling, spatial transcriptomics, and high-throughput imaging, as well as ...read more
Related Journals
![](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Small Heat Shock Proteins and the Endoplasmic Reticulum: Potential Attractive Therapeutic Targets?
Current Molecular Medicine Harnessing the Potential of Long Non-coding RNAs to Manage Metabolic Diseases
Current Pharmaceutical Design Molecular Bases of Chemoresistance in Cholangiocarcinoma
Current Drug Targets Evaluation of Tricine and EDDA as Co-ligands for <sup>99m</sup>Tc-Labeled HYNIC-MSH Analogs for Melanoma Imaging
Anti-Cancer Agents in Medicinal Chemistry Role of Metabolic Enzymes P450 (CYP) on Activating Procarcinogen and their Polymorphisms on the Risk of Cancers
Current Drug Metabolism Progress in Imaging Agents of Cell Apoptosis
Anti-Cancer Agents in Medicinal Chemistry Cytochrome P450-based Gene Therapy for Cancer Treatment: From Concept to the Clinic
Current Drug Metabolism Intracellular Trafficking of MDR Transporters and Relevance of SNPs
Current Topics in Medicinal Chemistry The Linkage Between Inflammation and Immune Tolerance: Interfering with Inflammation in Cancer
Current Cancer Drug Targets Synthesis and Preliminary Evaluation of 5-[18F]fluoroleucine
Current Radiopharmaceuticals Renal & Ocular Targets for Therapy in Wegeners Granulomatosis
Inflammation & Allergy - Drug Targets (Discontinued) AKT/GSK-3 Pathway Targeting; Botanicals and Bioactive Compounds with Anticancer Activities
Current Pharmaceutical Design Targeting the Lung: Preclinical and Comparative Evaluation of Anticancer Aerosols in Dogs with Naturally Occurring Cancers
Current Cancer Drug Targets Time Recall; Future Concept of Chronomodulating Chemotherapy for Cancer
Current Pharmaceutical Biotechnology Lung Cancer Epidemiology - A Global View
Current Respiratory Medicine Reviews Activated Rice Bran Supported Preyssler Heteropolyacid: A New, Green and Recyclable Biocomposite for Adsorption of Toxic Dyes
Current Nanoscience A Review of Depsipeptide and Other Histone Deacetylase Inhibitors in Clinical Trials
Current Pharmaceutical Design Occurrence and Severity of Adverse Reactions of Immune Checkpoint Inhibitors (PD-1 and PD L1) Based on Mordovian Dispensary Data Analysis
Current Cancer Therapy Reviews Clinical Pharmacology of Cyclophosphamide and Ifosfamide
Current Drug Therapy Nuclear Hormone Receptor Signals as New Therapeutic Targets for Urothelial Carcinoma
Current Cancer Drug Targets