Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

M.    Auxiliadora    Dea-Ayuela; Cristina       Galiana-Roselló; Aikaterini       Lalatsa; Dolores    R.    Serrano

doi:10.2174/1568026618666181025095735

Abstract

One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector- borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of resistance. Molecular assays based on Loop- Mediated Isothermal Amplification (LAMP) techniques are a good alternative to light microscopy and antigen-based rapid diagnostic tests in developing countries, since they allow for a large amount of genetic material generated from a few copies of DNA, and use primers that lead to high sensitivity and specificity, while the amplification process can be performed in isothermal conditions without the need of sophisticated equipment to interpret the results. In this review, the main advances in the development of LAMP assays for the diagnosis of malaria, leishmaniasis and Chagas' disease are discussed as well as the feasibility of their implementation in developing countries and use as point- of-care diagnostic tests.

Keywords: Loop-mediated isothermal amplification (LAMP), Leishmaniasis, American trypanosomiasis, Chagas disease, Malaria, diagnostic tools, Primers, Point-of care tests.

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Current Topics in Medicinal Chemistry

Title:Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

Volume: 18 Issue: 16

Author(s): M. Auxiliadora Dea-Ayuela*, Cristina Galiana-Roselló, Aikaterini Lalatsa and Dolores R. Serrano

Affiliation:

Departamento de Farmacia, Facultad de Ciencias de la Salud, Universidad Cardenal Herrera-CEU, Moncada, Valencia, 46113,Spain

Keywords: Loop-mediated isothermal amplification (LAMP), Leishmaniasis, American trypanosomiasis, Chagas disease, Malaria, diagnostic tools, Primers, Point-of care tests.

Abstract: One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector- borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of resistance. Molecular assays based on Loop- Mediated Isothermal Amplification (LAMP) techniques are a good alternative to light microscopy and antigen-based rapid diagnostic tests in developing countries, since they allow for a large amount of genetic material generated from a few copies of DNA, and use primers that lead to high sensitivity and specificity, while the amplification process can be performed in isothermal conditions without the need of sophisticated equipment to interpret the results. In this review, the main advances in the development of LAMP assays for the diagnosis of malaria, leishmaniasis and Chagas' disease are discussed as well as the feasibility of their implementation in developing countries and use as point- of-care diagnostic tests.

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Dea-Ayuela Auxiliadora M. *, Galiana-Roselló Cristina , Lalatsa Aikaterini and Serrano R. Dolores , Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs), Current Topics in Medicinal Chemistry 2018; 18 (16) . https://dx.doi.org/10.2174/1568026618666181025095735

DOI https://dx.doi.org/10.2174/1568026618666181025095735	Print ISSN 1568-0266
Publisher Name Bentham Science Publisher	Online ISSN 1873-4294

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