Abstract
RNA-binding proteins play critical roles in the regulation of gene expression. Among several families of RNA-binding proteins, PUF (Pumilio and FBF) proteins have been the subject of extensive investigations, as they can bind RNA in a sequence-specific manner and they are evolutionarily conserved among a wide range of organisms. The outstanding feature of these proteins is a highly conserved RNA-binding domain, which is known as the Pumilio-homology domain (PUM-HD) that mostly consists of eight tandem repeats. Each repeat recognizes an RNA base with a simple three-letter code that can be programmed in order to change the sequence-specificity of the protein. Using this tailored architecture, researchers have been able to change the specificity of the PUM-HD and target desired transcripts in the cell, even in subcellular compartments. The potential applications of this versatile tool in molecular cell biology seem unbounded and the use of these factors in pharmaceutics might be an interesting field of study in near future.
Keywords: RNA-binding proteins, PUF proteins, pumilio-homology domain, pumilio-binding element, gene expression, sequence- specificity.
Current Protein & Peptide Science
Title:PUF Proteins: Cellular Functions and Potential Applications
Volume: 18 Issue: 3
Author(s): Seyed Jalal Kiani, Tahereh Taheri, Sima Rafati and Katayoun Samimi-Rad
Affiliation:
Keywords: RNA-binding proteins, PUF proteins, pumilio-homology domain, pumilio-binding element, gene expression, sequence- specificity.
Abstract: RNA-binding proteins play critical roles in the regulation of gene expression. Among several families of RNA-binding proteins, PUF (Pumilio and FBF) proteins have been the subject of extensive investigations, as they can bind RNA in a sequence-specific manner and they are evolutionarily conserved among a wide range of organisms. The outstanding feature of these proteins is a highly conserved RNA-binding domain, which is known as the Pumilio-homology domain (PUM-HD) that mostly consists of eight tandem repeats. Each repeat recognizes an RNA base with a simple three-letter code that can be programmed in order to change the sequence-specificity of the protein. Using this tailored architecture, researchers have been able to change the specificity of the PUM-HD and target desired transcripts in the cell, even in subcellular compartments. The potential applications of this versatile tool in molecular cell biology seem unbounded and the use of these factors in pharmaceutics might be an interesting field of study in near future.
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Cite this article as:
Kiani Jalal Seyed, Taheri Tahereh, Rafati Sima and Samimi-Rad Katayoun, PUF Proteins: Cellular Functions and Potential Applications, Current Protein & Peptide Science 2017; 18 (3) . https://dx.doi.org/10.2174/1389203717666160914172908
DOI https://dx.doi.org/10.2174/1389203717666160914172908 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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