摘要
背景:目前的时代,在基因组编辑技术方面快速创新。 CRISPR Cas9介导的靶向遗传操作是一种简单,成本效益和可扩展的方法。因此,它可用于广泛的靶向基因组工程。 目的:本次审查的主要目的是突出基因组学编辑工具包的结构特征,分类及其基础科学与医学应用,以及未来的挑战。 结果:本综述简要介绍了CRISPR-Cas9基因组编辑技术近期发展。我们讨论下一代基因组编辑技术CRISPR的范式转变。 CRISPR的结构意义,分类及其不同应用也在讨论中。我们描绘了这个非凡的基因组体内编辑工具的未来挑战。我们还强调CRISPR基因组编辑在治愈许多疾病中的作用。 结论:科学家和研究人员正在不断地寻找一种基因组编辑工具,它是能量,简单和低成本组装的核酸酶。它可以靶向任何特定的位点,而不会在基因组中发生任何脱靶突变。 CRISPR-Cas9具有以上所有特点。基因组工程技术可能是下一代药物开发的强大和鼓舞人心的技术。
关键词: 阿尔茨海默病,痴呆,药物递送,纳米颗粒,神经保护,神经原纤维缠结。
Current Drug Targets
Title:The Smart Programmable CRISPR Technology: A Next Generation Genome Editing Tool for Investigators
Volume: 18 Issue: 14
关键词: 阿尔茨海默病,痴呆,药物递送,纳米颗粒,神经保护,神经原纤维缠结。
摘要: Background: The present era is fast experiencing rapid innovation in the genome-editing technology. CRISPR Cas9-mediated targeted genetic manipulation is an easy, cost-effective and scalable method. As a result, it can be used for a broad range of targeted genome engineering.
Objective: The main objective of the present review is to highlight the structural signature, classification, its mechanism and application from basic science to medicine and future challenges for this genome editing tool kit.
Results: The present review provides a brief description of the recent development of CRISPR-Cas9 genome editing technology. We discuss the paradigms shift for this next generation genome editing technology, CRISPR. The CRISPR structural significance, classification and its different applications are also being discussed. We portray the future challenges for this extraordinary genome in vivo editing tool. We also highlight the role of CRISPR genome editing in curing many diseases.
Conclusion: Scientists and researchers are constantly looking one genome editing tool that is competent, simple and low-cost assembly of nucleases. It can target any particular site without any off-target mutations in the genome. The CRISPR-Cas9 has all of the above characteristics. The genome engineering technology may be a strong and inspiring technology meant for the next generation of drug development.
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Cite this article as:
The Smart Programmable CRISPR Technology: A Next Generation Genome Editing Tool for Investigators, Current Drug Targets 2017; 18 (14) . https://dx.doi.org/10.2174/1389450117666160527142321
DOI https://dx.doi.org/10.2174/1389450117666160527142321 |
Print ISSN 1389-4501 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5592 |
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