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Abstract
Thrombin, a highly specific protease of blood coagulation, has two exosites that modulate its specificity. We designed two sets of synthetic substrate FRET peptides with 25- or 11- amino acids (aa) each, based on the PAR 1 sequence, to characterize the effect of exosite 1 engagement on substrate catalysis and preference. The 25-aa set encompassed a sequence binding to exosite 1, and structural modeling showed that binding to thrombin did not differ significantly from that of PAR 1 peptide. Modification at the P3´position of the 25 or 11-aa peptides resulted in small effect on kinetic parameters. Ionic strength higher than physiologic depressed thrombin action on the 25-aa peptides. Addition of ligands of the exosite 1 negatively modulated the catalysis of 25-aa substrates. In conclusion, we succeeded to mimic and study in real time, using these synthetic peptides, the influence of ligand binding to exosite 1 on thrombin activity.
Keywords: Enzyme activity, exosite 1, fluorogenic peptides, PAR 1 and thrombin.
Protein & Peptide Letters
Title:Investigation of Thrombin Activity with PAR 1-based Fluorogenic Peptides
Volume: 20 Issue: 10
Author(s): Saulo Martins Vieira, Flavia Garcia dos Reis, Reinaldo Geraldo, Denis Luis da Silva Dutra, Luiz Juliano, Maria Aparecida Julianod, Julio Alberto Mignaco and Russolina Benedeta Zingali
Affiliation:
Keywords: Enzyme activity, exosite 1, fluorogenic peptides, PAR 1 and thrombin.
Abstract: Thrombin, a highly specific protease of blood coagulation, has two exosites that modulate its specificity. We designed two sets of synthetic substrate FRET peptides with 25- or 11- amino acids (aa) each, based on the PAR 1 sequence, to characterize the effect of exosite 1 engagement on substrate catalysis and preference. The 25-aa set encompassed a sequence binding to exosite 1, and structural modeling showed that binding to thrombin did not differ significantly from that of PAR 1 peptide. Modification at the P3´position of the 25 or 11-aa peptides resulted in small effect on kinetic parameters. Ionic strength higher than physiologic depressed thrombin action on the 25-aa peptides. Addition of ligands of the exosite 1 negatively modulated the catalysis of 25-aa substrates. In conclusion, we succeeded to mimic and study in real time, using these synthetic peptides, the influence of ligand binding to exosite 1 on thrombin activity.
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Vieira Martins Saulo, Reis Garcia dos Flavia, Geraldo Reinaldo, Dutra Luis da Silva Denis, Juliano Luiz, Julianod Aparecida Maria, Mignaco Alberto Julio and Zingali Benedeta Russolina, Investigation of Thrombin Activity with PAR 1-based Fluorogenic Peptides, Protein & Peptide Letters 2013; 20 (10) . https://dx.doi.org/10.2174/09298665113209990001
DOI https://dx.doi.org/10.2174/09298665113209990001 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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