Abstract
Marine algae can serve as sources of bioactive compounds and currently have been shown their potential biological and pharmaceutical applications. Marine algae lectins have been shown to be effective at controlling inflammatory processes. This work aimed to analyze the immunostimulatory properties of lectins from the marine algae Solieria filiformis (SfL), Pterocladiella capillacea (PcL) and Caulerpa cupressoides (CcL). This analysis was performed on BALB/c mouse splenocytes by measuring cytokine and nitric oxide production and cellular damage using tests of cytotoxicity and cell viability. These lectins were not cytotoxic (1-100 μg/mL), and were not able to induce IFN-γ and IL-2 production. IL- 10 production was induced at high levels by all lectins tested. Treatment with SfL induced IL-6 production at higher levels at all experimental times, whereas treatment with PcL and CcL induced higher levels only in 24 and 72 h. Treatment with SfL did not result in nitrite oxide production, whereas treatment with PcL or CcL was able to induce nitrite release at high levels (after 24, 48 and 72 h). Lesser cellular damage (5%) was observed in splenocytes treated with these lectins (10 μg/mL). Thus, the lectins from these algae were not cytotoxic, promoted increased in cell viability and induced Th2 immune responses in mouse splenocytes, indicating that they have anti-inflammatory effects.
Keywords: Cytokines, cytoxicity, immunomodulation, lectins, marine algae, nitric oxide
Protein & Peptide Letters
Title:Cytokine Production Induced by Marine Algae Lectins in BALB/c Mice Splenocytes
Volume: 19 Issue: 9
Author(s): Ticiana Monteiro Abreu, Luana Maria Castelo Melo Silva, Edfranck Sousa Oliveira Vanderlei, Cristiane Moutinho Lagos de Melo, Valeria Rego Alves Pereira and Norma Maria Barros Benevides
Affiliation:
Keywords: Cytokines, cytoxicity, immunomodulation, lectins, marine algae, nitric oxide
Abstract: Marine algae can serve as sources of bioactive compounds and currently have been shown their potential biological and pharmaceutical applications. Marine algae lectins have been shown to be effective at controlling inflammatory processes. This work aimed to analyze the immunostimulatory properties of lectins from the marine algae Solieria filiformis (SfL), Pterocladiella capillacea (PcL) and Caulerpa cupressoides (CcL). This analysis was performed on BALB/c mouse splenocytes by measuring cytokine and nitric oxide production and cellular damage using tests of cytotoxicity and cell viability. These lectins were not cytotoxic (1-100 μg/mL), and were not able to induce IFN-γ and IL-2 production. IL- 10 production was induced at high levels by all lectins tested. Treatment with SfL induced IL-6 production at higher levels at all experimental times, whereas treatment with PcL and CcL induced higher levels only in 24 and 72 h. Treatment with SfL did not result in nitrite oxide production, whereas treatment with PcL or CcL was able to induce nitrite release at high levels (after 24, 48 and 72 h). Lesser cellular damage (5%) was observed in splenocytes treated with these lectins (10 μg/mL). Thus, the lectins from these algae were not cytotoxic, promoted increased in cell viability and induced Th2 immune responses in mouse splenocytes, indicating that they have anti-inflammatory effects.
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Monteiro Abreu Ticiana, Maria Castelo Melo Silva Luana, Sousa Oliveira Vanderlei Edfranck, Moutinho Lagos de Melo Cristiane, Rego Alves Pereira Valeria and Maria Barros Benevides Norma, Cytokine Production Induced by Marine Algae Lectins in BALB/c Mice Splenocytes, Protein & Peptide Letters 2012; 19 (9) . https://dx.doi.org/10.2174/092986612802084474
DOI https://dx.doi.org/10.2174/092986612802084474 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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