Abstract
Mycobacterium tuberculosis encounters a range of stimuli in the host. Understanding the environmental cues that initiate the transcriptional response of M. tuberculosis, which enable the bacterium to replicate and/or survive in the host, will provide markers that are specific to different stages of disease, further refining the search for improved treatments and vaccines. Studying M. tuberculosis gene expression in vivo is technically challenging and more amenable in vitro experiments are being used to aid interpretation and to dissect the signals that are responsible for controlling subsets of genes. Key parameters that affect the growth of a pathogen in the host include nutrient status, environmental pH, oxygen availability, and host defences. Studying gene expression, pathogenicity, and physiology of M. tuberculosis that has been exposed to these relevant host conditions in vitro will further increase our understanding of the virulence factors that M. tuberculosis requires to establish disease. Complementary information obtained by metabolic flux analysis, proteomics, and regulatory networks analysis will enable a clearer picture of how transcriptional responses translate to changes in the metabolome and physiology of the organism.
Keywords: Latency, chemostat, iron-limitation, regulation, hypoxia, nitric oxide, carbon starvation, re-activation
Current Molecular Medicine
Title: Transcriptional Responses of Mycobacterium tuberculosis Exposed to Adverse Conditions In Vitro
Volume: 7 Issue: 3
Author(s): Joanna Bacon and Philip D. Marsh
Affiliation:
Keywords: Latency, chemostat, iron-limitation, regulation, hypoxia, nitric oxide, carbon starvation, re-activation
Abstract: Mycobacterium tuberculosis encounters a range of stimuli in the host. Understanding the environmental cues that initiate the transcriptional response of M. tuberculosis, which enable the bacterium to replicate and/or survive in the host, will provide markers that are specific to different stages of disease, further refining the search for improved treatments and vaccines. Studying M. tuberculosis gene expression in vivo is technically challenging and more amenable in vitro experiments are being used to aid interpretation and to dissect the signals that are responsible for controlling subsets of genes. Key parameters that affect the growth of a pathogen in the host include nutrient status, environmental pH, oxygen availability, and host defences. Studying gene expression, pathogenicity, and physiology of M. tuberculosis that has been exposed to these relevant host conditions in vitro will further increase our understanding of the virulence factors that M. tuberculosis requires to establish disease. Complementary information obtained by metabolic flux analysis, proteomics, and regulatory networks analysis will enable a clearer picture of how transcriptional responses translate to changes in the metabolome and physiology of the organism.
Export Options
About this article
Cite this article as:
Bacon Joanna and Marsh D. Philip, Transcriptional Responses of Mycobacterium tuberculosis Exposed to Adverse Conditions In Vitro, Current Molecular Medicine 2007; 7 (3) . https://dx.doi.org/10.2174/156652407780598566
DOI https://dx.doi.org/10.2174/156652407780598566 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
![](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Occurrence and Biological Properties of Sphingolipids - A Review
Current Nutrition & Food Science Exploring and Exploiting Gene Networks That Regulate Natural Products Biosynthesis in Actinobacteria
The Natural Products Journal Bacterial Resistance: Antibiotics of Last Generation used in Clinical Practice and the Arise of Natural Products as New Therapeutic Alternatives
Current Pharmaceutical Design Meet the Editorial Board Member
Current Pharmaceutical Biotechnology Transition Towards Antibiotic Hybrid Vehicles: The Next Generation Antibacterials
Current Medicinal Chemistry Molecular Targets for Design of Novel Inhibitors to Circumvent Aminoglycoside Resistance
Current Drug Targets Understanding the Structure, Activity and Inhibition of Chorismate Synthase from Mycobacterium tuberculosis
Current Medicinal Chemistry Mouse Models of Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis
Current Pharmaceutical Design Prediction of Nicotinamide Adenine Dinucleotide Interacting Sites Based on Ensemble Support Vector Machine
Protein & Peptide Letters Mechanisms of Action and Chemical-Biological Interactions Between Ozone and Body Compartments: A Critical Appraisal of the Different Administration Routes
Current Drug Therapy Vitamins Mediate Immunological Homeostasis and Diseases at the Surface of the Body
Endocrine, Metabolic & Immune Disorders - Drug Targets Adverse Effects of Statins - Mechanisms and Consequences
Current Drug Safety Role of Type I Cytokines in Host Defense Against Mycobacterium avium Infection
Current Pharmaceutical Design Monocytes and Macrophages as Cellular Targets in Liver Fibrosis
Inflammation & Allergy - Drug Targets (Discontinued) Multifunctional Nanoparticles, Nanocages and Degradable Polymers as a Potential Novel Generation of Non-Invasive Molecular and Cellular Imaging Systems
Recent Patents on Nanotechnology Microarray Analysis of Whole Genome Expression of Intracellular Mycobacterium tuberculosis
Current Molecular Medicine Oligonucleotide Profiling for Discriminating Bacteria in Bacterial Communities
Combinatorial Chemistry & High Throughput Screening Invasive Aspergillosis in Children and Adolescents
Current Pharmaceutical Design The Implication of Pseudomonas aeruginosa Biofilms in Infections
Inflammation & Allergy - Drug Targets (Discontinued) Structure- and Ligand-Based Structure-Activity Relationships for a Series of Inhibitors of Aldolase
Current Computer-Aided Drug Design