Isolation and Characterization of an Angiogeninlike Protein from Goat Plasma

Title: Isolation and Characterization of an Angiogeninlike Protein from Goat Plasma

Volume: 9 Issue: 4

Author(s): Tushar Kanti Maiti and Swagata Dasgupta

Affiliation:

Keywords: Angiogeninlike Protei, Goat Plasma, Ribonuclease Inhibitor, chorioallantoic membrane

Abstract: Angiogenin, a blood vessel inducing protein has been implicated in wound healing and tumour progression. First isolated from human carcinoma cells, it has been subsequently isolated from human, bovine, rabbit, pig and mouse sera and bovine milk. This study reports the isolation of an angiogenic-like protein from goat plasma. The ribonucleolytic activity has been followed by yeast transfer RNA (tRNA) degradation using spectrophotometric and denaturing polyacrylamide gel electrophoresis methods. The chorioallantoic membrane (CAM) assay has been implemented to study its angiogenic activity. The presence of this protein has also been confirmed by strong binding with placental Ribonuclease Inhibitor (PRI).

Cite this article as:

Maiti Kanti Tushar and Dasgupta Swagata, Isolation and Characterization of an Angiogeninlike Protein from Goat Plasma, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408661