Abstract
Formylpeptide receptors are well-characterized receptors which participate in host defense responses of neutrophils. We designed and synthesized chemotactic peptide analog with pbenzoylphenylalanine (Bpa) and biotin to probe structural and mechanistic aspects of peptide-receptor interaction. These peptides possess biological activities which were dependent upon spacer residue length of and Bpa position. The covalent photoaffinity label was detected by Streptavidine-blot, which was inhibited by the parent peptide.
Keywords: chemotactic peptide, fmlp, formylpeptide receptor, photoaffinity labeling, cross-linking, p-benzoylphenylalanine, affinity tag, biotin
Protein & Peptide Letters
Title: Synthesis of Chemotactic Peptide Analogs with a Photoaffinity Cross-Linker as New Probes for Analysis of Receptor-Ligand Interaction
Volume: 10 Issue: 2
Author(s): Mitsukuni Shibue, Masahiro Yoshiki, Masaya Miyazaki, Ichiro Fujita, Satoshi Osada, Seiji Yasuda, Hiroyuki Nakamura, Yuhei Hamasaki, Michio Kondo and Hideaki Maeda
Affiliation:
Keywords: chemotactic peptide, fmlp, formylpeptide receptor, photoaffinity labeling, cross-linking, p-benzoylphenylalanine, affinity tag, biotin
Abstract: Formylpeptide receptors are well-characterized receptors which participate in host defense responses of neutrophils. We designed and synthesized chemotactic peptide analog with pbenzoylphenylalanine (Bpa) and biotin to probe structural and mechanistic aspects of peptide-receptor interaction. These peptides possess biological activities which were dependent upon spacer residue length of and Bpa position. The covalent photoaffinity label was detected by Streptavidine-blot, which was inhibited by the parent peptide.
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Cite this article as:
Shibue Mitsukuni, Yoshiki Masahiro, Miyazaki Masaya, Fujita Ichiro, Osada Satoshi, Yasuda Seiji, Nakamura Hiroyuki, Hamasaki Yuhei, Kondo Michio and Maeda Hideaki, Synthesis of Chemotactic Peptide Analogs with a Photoaffinity Cross-Linker as New Probes for Analysis of Receptor-Ligand Interaction, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479031
DOI https://dx.doi.org/10.2174/0929866033479031 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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