Analysis of Seven Terpenoids by HS-SPME Coupled with GC-MS for the Identification and Classification of Different Teas

Title:Analysis of Seven Terpenoids by HS-SPME Coupled with GC-MS for the Identification and Classification of Different Teas

Volume: 20 Issue: 9

Author(s): Yangzhou Xie, Yi Yang, Yu Tian, Zhimin Liu, Zhigang Xu*, Wei Jiang, Zhihua Liu and Xiaoxi Si*

Affiliation:

• Department of Chemistry, Faculty of Science, Kunming University of Science and Technology, Kunming, 650500, China;

• Yunnan Key Laboratory of Tobacco Chemistry, R&D Center of China Tobacco Yunnan Industrial Co., Ltd., Kunming, 650231, China
• Faculty of Environmental Science & Engineering, Kunming University of Science and Technology, Kunming, 650500, China

Keywords: Terpenoids, chiral enantiomers, headspace solid-phase microextraction, gas chromatography-mass spectrometry, tea, principal component analysis.

Abstract:

Background: Terpenoids are essential aroma substances in teas, and their concentration brings various characteristics to different teas. Therefore, developing a simple and stable method is necessary for distinguishing tea categories.

Objective: In previous studies, more attention was paid to non-chiral isomers of terpenes due to the challenges of separating chiral isomers. So, this paper aims to present a method for effectively separating seven terpenoid substances, including chiral isomers and non-chiral isomers, to facilitate the classification and identification of teas.

Methods: A method utilizing headspace solid-phase microextraction coupled with gas chromatography- mass spectrometry was used to isolate and analyze 7 terpenoid compounds. After optimized conditions, the BGB-176 chiral column and the PDMS/DVB fiber were selected for subsequent analysis.

Results: This method has a good linear range of 0.1-200 mg/L, and its linear correlation coefficients are between 0.9974 and 0.9994, and the limit of detection and the limit of quantification is 0.02–0.03 and 0.06–0.09 mg/L, respectively. Only five terpenoid substances were detected in a total of 15 tea samples. Furthermore, In the detection of carvon and α-ionone optical isomers, the S isomer was mainly detected.

Conclusions: An effective approach was developed to separate and analyze 7 terpenoid compounds in natural and synthetic teas. Meanwhile, 15 tea samples can be identified and classified using principal component analysis.

Cite this article as:

Xie Yangzhou, Yang Yi, Tian Yu, Liu Zhimin, Xu Zhigang*, Jiang Wei, Liu Zhihua and Si Xiaoxi*, Analysis of Seven Terpenoids by HS-SPME Coupled with GC-MS for the Identification and Classification of Different Teas, Current Analytical Chemistry 2024; 20 (9) . https://dx.doi.org/10.2174/0115734110301044240426170020