Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead

Title:Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead

Volume: 25 Issue: 17

Author(s): Henrique Bonifácio Moura, Henrique Gama Ker, Luana S. Ramos, Lais M. Nogueira, Juliana M. Machado, Daniel S. Dias, Rita Alaíde Leandro Rodrigues, Mariana Campos da Paz, Rafael Gonçalves Teixeira-Neto, Alexandre Barbosa Reis, Alexsandro S. Galdino*Eduardo S. Silva*

Affiliation:

• Laboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil

• Laboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501- 296, Brazil

Keywords: Leishmania infantum, canine visceral leishmaniasis, diagnosis, flow cytometry serology, dogs, zoonotic disease

Abstract:

Background: Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite.

Objective: To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis.

Methods: The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH.

Results: In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs’ clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity.

Conclusion: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.

Cite this article as:

Moura Bonifácio Henrique, Ker Gama Henrique, Ramos S. Luana, Nogueira M. Lais, Machado M. Juliana, Dias S. Daniel, Rodrigues Alaíde Leandro Rita, Paz Campos da Mariana, Teixeira-Neto Gonçalves Rafael, Reis Barbosa Alexandre, Galdino S. Alexsandro*, Silva S. Eduardo*, Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead, Current Pharmaceutical Biotechnology 2024; 25 (17) . https://dx.doi.org/10.2174/0113892010268142231226115140