Login Register Cart 0
Generic placeholder image

Current Aging Science

Editor-in-Chief

ISSN (Print): 1874-6098
ISSN (Online): 1874-6128

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Neuroprotective Effects of Curcumin against Chronic Chlorpyrifos- Induced Oxidative Damage in Rat Brain Tissue

Author(s): Tahereh Farkhondeh*, Mahmoud Zardast, Shahnaz Rajabi, Mahdi Abdollahi-Karizno, Babak Roshanravan, Jalal Havangi, Michael Aschner and Saeed Samarghandian*

Volume 17, Issue 3, 2024

Published on: 12 February, 2024

Page: [205 - 209] Pages: 5

DOI: 10.2174/0118746098244014240119112706

Price: $65

Abstract

Background: Chlorpyrifos (CPF) is an organophosphate pesticide that inhibits acetylcholinesterase (AChE) activity. Investigations have also focused on its neurotoxicity, which is independent of AChE inhibition. Here, we evaluated the effect of CPF on oxidative indices in the brain tissue and explored the protective effect of curcumin (Cur) against its toxicity.

Methods: Forty male Wistar rats were divided into five groups, each consisting of eight rats (n = 8) per group. Animals were administrated by oral gavage for 90 days with the following treatments: control (C), CPF, CPF + CUR 25 mg/kg, CPF + CUR50, and CPF + cur 100 received olive oil, CPF, CPF plus 25 mg/kg of CUR, CPF plus 50 mg/kg of CUR, and CPF plus 100 mg/kg of CUR, respectively. After anesthetization, animal brain tissues were obtained for assessment of oxidative stress indices.

Results: The concentration of MDA significantly increased in the brains of the CPF group as compared to the control group (p < 0.01). Also, a significant decrease in MDA concentrations was observed in the brains of rats in the CPF + Cur 100 group compared to the CPF group (p < 0.05). A significant decrease was noted in the GSH concentration in the brains of the CPF group compared to the control group (p < 0.05). Treatment with Cur at 100 mg/kg exhibited a significant increase in GSH concentrations in the brains of the CPF-exposed group compared to the CPF group without Cur administration (p < 0.05). The concentration of NO exhibited a significant increase in the brains of the CPF group when compared to the control group (p < 0.05). Also, a significant decrease in NO concentration was observed in the brain tissue of the CPF + Cur 100 group compared to the CPF group (p < 0.05).

Conclusion: Our data establish that chronic exposure to CPF induced oxidative stress in brain tissue, which was reversed by CUR administration. Additional experimental and clinical investigations are needed to validate the efficacy of CUR as a potential antidote for CPF poisoning.

Keywords: Chlorpyrifos, curcumin, oxidative stress, brain, organphosphate pesticides, AChE.

« Previous Next »
Graphical Abstract

[1]
El-Nahhal Y, El-Nahhal I. Cardiotoxicity of some pesticides and their amelioration. Environ Sci Pollut Res Int 2021; 28(33): 44726-54.
[http://dx.doi.org/10.1007/s11356-021-14999-9] [PMID: 34231153]
[2]
Bhattu M, Verma M, Kathuria D. Recent advancements in the detection of organophosphate pesticides: A review. Anal Methods 2021; 13(38): 4390-428.
[http://dx.doi.org/10.1039/D1AY01186C] [PMID: 34486591]
[3]
Nandhini AR, Harshiny M, Gummadi SN. Chlorpyrifos in environment and food: A critical review of detection methods and degradation pathways. Environ Sci Process Impacts 2021; 23(9): 1255-77.
[http://dx.doi.org/10.1039/D1EM00178G] [PMID: 34553733]
[4]
Khokhar JY, Tyndale RF. Rat brain CYP2B-enzymatic activation of chlorpyrifos to the oxon mediates cholinergic neurotoxicity. Toxicol Sci 2012; 126(2): 325-35.
[http://dx.doi.org/10.1093/toxsci/kfs029] [PMID: 22287024]
[5]
Nguyen A, Patel AB, Kioutchoukova IP, Diaz MJ, Lucke-Wold B. Mechanisms of mitochondrial oxidative stress in brain injury: From pathophysiology to therapeutics. Oxygen 2023; 3(2): 163-78.
[http://dx.doi.org/10.3390/oxygen3020012] [PMID: 37082315]
[6]
Logsdon AF, Lucke-Wold BP, Nguyen L, et al. Salubrinal reduces oxidative stress, neuroinflammation and impulsive-like behavior in a rodent model of traumatic brain injury. Brain Res 2016; 1643: 140-51.
[http://dx.doi.org/10.1016/j.brainres.2016.04.063] [PMID: 27131989]
[7]
Kaur S, Singla N, Dhawan DK. Neuro-protective potential of quercetin during chlorpyrifos induced neurotoxicity in rats. Drug Chem Toxicol 2019; 42(2): 220-30.
[http://dx.doi.org/10.1080/01480545.2019.1569022] [PMID: 30747009]
[8]
Feng K, Ge Y, Chen Z, et al. Curcumin inhibits the PERK-eIF2α-CHOP pathway through promoting SIRT1 expression in oxidative stress-induced rat chondrocytes and ameliorates osteoarthritis progression in a rat model. Oxid Med Cell Longev 2019; 2019: 1-17.
[http://dx.doi.org/10.1155/2019/8574386]
[9]
Huang L, Li X, Liu Y, et al. Curcumin alleviates cerebral ischemia-reperfusion injury by inhibiting NLRP1-dependent neuronal pyroptosis. Curr Neurovasc Res 2021; 18(2): 189-96.
[http://dx.doi.org/10.2174/1567202618666210607150140] [PMID: 34109908]
[10]
Wolkmer P, da Silva CB, Paim FC, et al. Pre-treatment with curcumin modulates acetylcholinesterase activity and proinflammatory cytokines in rats infected with Trypanosoma evansi. Parasitol Int 2013; 62(2): 144-9.
[http://dx.doi.org/10.1016/j.parint.2012.11.004] [PMID: 23200738]
[11]
Orlando RA, Gonzales AM, Royer RE, Deck LM, Vander Jagt DL. A chemical analog of curcumin as an improved inhibitor of amyloid Abeta oligomerization. PLoS One 2012; 7(3): e31869.
[http://dx.doi.org/10.1371/journal.pone.0031869] [PMID: 22442659]
[12]
Naughton SX, Terry AV Jr. Neurotoxicity in acute and repeated organophosphate exposure. Toxicology 2018; 408: 101-12.
[http://dx.doi.org/10.1016/j.tox.2018.08.011] [PMID: 30144465]
[13]
Sobolev VE, Sokolova MO, Jenkins RO, Goncharov NV. Molecular mechanisms of acute organophosphate nephrotoxicity. Int J Mol Sci 2022; 23(16): 8855.
[http://dx.doi.org/10.3390/ijms23168855] [PMID: 36012118]
[14]
Guignet M, Dhakal K, Flannery BM, et al. Persistent behavior deficits, neuroinflammation, and oxidative stress in a rat model of acute organophosphate intoxication. Neurobiol Dis 2020; 133: 104431.
[http://dx.doi.org/10.1016/j.nbd.2019.03.019] [PMID: 30905768]
[15]
Liang LP, Pearson-Smith JN, Huang J, McElroy P, Day BJ, Patel M. Neuroprotective effects of AEOL10150 in a rat organophosphate model. Toxicol Sci 2018; 162(2): 611-21.
[http://dx.doi.org/10.1093/toxsci/kfx283] [PMID: 29272548]
[16]
Čolović MB, Vasić VM, Avramović NS, Gajić MM, Djurić DM, Krstić DZ. In vitroevaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. Toxicol Lett 2015; 233(1): 29-37.
[http://dx.doi.org/10.1016/j.toxlet.2015.01.003] [PMID: 25562544]
[17]
Mahmoud SM, Abdel Moneim AE, Qayed MM, El-Yamany NA. Potential role of N-acetylcysteine on chlorpyrifos-induced neurotoxicity in rats. Environ Sci Pollut Res Int 2019; 26(20): 20731-41.
[http://dx.doi.org/10.1007/s11356-019-05366-w] [PMID: 31104238]
[18]
Taha MAI, Badawy MEI, Abdel-Razik RK, Younis HM, Abo-El-Saad MM. Mitochondrial dysfunction and oxidative stress in liver of male albino rats after exposing to sub-chronic intoxication of chlorpyrifos, cypermethrin, and imidacloprid. Pestic Biochem Physiol 2021; 178: 104938.
[http://dx.doi.org/10.1016/j.pestbp.2021.104938] [PMID: 34446205]
[19]
Sies H. Oxidative stress: A concept in redox biology and medicine. Redox Biol 2015; 4: 180-3.
[http://dx.doi.org/10.1016/j.redox.2015.01.002] [PMID: 25588755]
[20]
El-Demerdash FM. Oxidative stress and hepatotoxicity induced by synthetic pyrethroids-organophosphate insecticides mixture in rat. J Environ Sci Health Part C Environ Carcinog Ecotoxicol Rev 2011; 29(2): 145-58.
[http://dx.doi.org/10.1080/10590501.2011.577679] [PMID: 21660820]
[21]
Selmi S, El-Fazaa S, Gharbi N. Oxidative stress and alteration of biochemical markers in liver and kidney by malathion in rat pups. Toxicol Ind Health 2015; 31(9): 783-8.
[http://dx.doi.org/10.1177/0748233713475507] [PMID: 23344821]
[22]
Fortunato JJ, Feier G, Vitali AM, Petronilho FC, Dal-Pizzol F, Quevedo J. Malathion-induced oxidative stress in rat brain regions. Neurochem Res 2006; 31(5): 671-8.
[http://dx.doi.org/10.1007/s11064-006-9065-3] [PMID: 16770738]
[23]
Lucke-Wold BP, Naser ZJ, Logsdon AF, et al. Amelioration of nicotinamide adenine dinucleotide phosphate–oxidase mediated stress reduces cell death after blast-induced traumatic brain injury. Transl Res 2015; 166(6): 509-528.e1.
[http://dx.doi.org/10.1016/j.trsl.2015.08.005] [PMID: 26414010]
[24]
Aghili-Mehrizi S, Williams E, Yan S, Willman M, Willman J, Lucke-Wold B. Secondary mechanisms of neurotrauma: A closer look at the evidence. Diseases 2022; 10(2): 30.
[http://dx.doi.org/10.3390/diseases10020030] [PMID: 35645251]
[25]
El-Ebiary AA, Elsharkawy RE, Soliman NA, Soliman MA, Hashem AA. N‐acetylcysteine in acute organophosphorus pesticide poisoning: A randomized, clinical trial. Basic Clin Pharmacol Toxicol 2016; 119(2): 222-7.
[http://dx.doi.org/10.1111/bcpt.12554] [PMID: 26786042]
[26]
Cankayali L, Demirag K, Eris O, Ersoz B, Moral AR. The effects ofN-acetylcysteine on oxidative stress in organophosphate poisoning model. Adv Ther 2005; 22(2): 107-16.
[http://dx.doi.org/10.1007/BF02849882] [PMID: 16020401]
[27]
John JJ, Nagar DP, Gujar NL, Bhattacharya R. Oxidative and histopathological alterations after sub-acute exposure of diisopropyl phosphorofluoridate in mice: Beneficial effect of N acetylcysteine. Life Sci 2019; 228: 98-111.
[http://dx.doi.org/10.1016/j.lfs.2019.04.067] [PMID: 31051153]
[28]
Uzunhisarcikli M, Kalender Y. Protective effects of vitamins C and E against hepatotoxicity induced by methyl parathion in rats. Ecotoxicol Environ Saf 2011; 74(7): 2112-8.
[http://dx.doi.org/10.1016/j.ecoenv.2011.07.001] [PMID: 21782244]
[29]
Fereidouni S, Kumar RR, Chadha VD, Dhawan DK. Quercetin plays protective role in oxidative induced apoptotic events during chronic chlorpyrifos exposure to rats. J Biochem Mol Toxicol 2019; 33(8): e22341.
[http://dx.doi.org/10.1002/jbt.22341] [PMID: 30990955]
[30]
Ibrahim KA, Eleyan M, Abd El-Rahman HA, Khwanes SA, Mohamed RA, Mohamed RA. Quercetin attenuates the oxidative injury–mediated upregulation of apoptotic gene expression and catecholaminergic neurotransmitters of the fetal rats’ brain following prenatal exposure to fenitrothion insecticide. Neurotox Res 2020; 37(4): 871-82.
[http://dx.doi.org/10.1007/s12640-020-00172-6] [PMID: 32034696]
[31]
Karami-Mohajeri S, Najafi A, Behnam B, et al. Protective effect of Zataria multiflora Boiss. and its main compound, rosmarinic acid, against malathion induced oxidative stress and apoptosis in HepG2 cells. J Environ Sci Health B 2021; 56(4): 297-306.
[http://dx.doi.org/10.1080/03601234.2021.1879595] [PMID: 33560903]
[32]
Baldissera MD, Souza CF, Viana AR, da Silva AS, Baldisserotto B. Protective role of rutin dietary supplementation mediated by purinergic signaling in spleen of silver catfish Rhamdia quelen exposed to organophosphate pesticide trichlorfon. Comp Biochem Physiol C Toxicol Pharmacol 2021; 244: 109006.
[http://dx.doi.org/10.1016/j.cbpc.2021.109006] [PMID: 33610818]

Rights & Permissions Print Cite
Article Metrics
6
1
Wayfinder Image
© 2024 Bentham Science Publishers | Privacy Policy