Abstract
Objective: The aim of this study was to construct a multicompartment synchronous rotating bioreactor (MCSRB) for batch-production of homogenized adipose-derived stem cell (ADSC) microspheres and treat neurogenic erectile dysfunction (ED).
Methods: Firstly, an MCSRB was constructed using a centrifugal device and hinged trays. Secondly, influence factors (density, rotational speed) on the formation of ADSC-spheroids were explored. Finally, a neurogenic ED model was established to verify the effectiveness and safety of ADSC-spheroids for ED treatment.
Results: An MCSRB promoted ADSCs to gather microspheres, most of which were 90-130 μm in diameter. Supernatant from three-dimensional culture led to a significant increase in cytokine expression in ADSCs and migration rate in human umbilical vein endothelial cells (HUVECs) compared to control groups. The erectile function and pathological changes of the penis were improved in the ADSC-spheroids treatment group compared to the traditional ADSCs treatment group (p < 0.01).
Conclusion: Efficient, batch, controlled and homogenized production of ADSC stem cell microspheres, and effective improvement of erectile dysfunction in neurogenic rats can be achieved using the MCSRB device.
Keywords: Centrifugation culture, adipose-derived stem cells (ADSCs), stem cell microspheres, erectile dysfunction, cavernous nerve damage.
Current Stem Cell Research & Therapy
Title:A Novel Multi-compartment Rotating Bioreactor for Improving ADSC-Spheroid Formation and its Application in Neurogenic Erectile Dysfunction
Volume: 19 Issue: 10
Author(s): Peng Wang, Yang Liu, Xiao-Feng Duan, Xiao-Ying Pan, Xiang-Rui Kong and Yong Yang*
Affiliation:
- Department of Urology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, China
- Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China
Keywords: Centrifugation culture, adipose-derived stem cells (ADSCs), stem cell microspheres, erectile dysfunction, cavernous nerve damage.
Abstract:
Objective: The aim of this study was to construct a multicompartment synchronous rotating bioreactor (MCSRB) for batch-production of homogenized adipose-derived stem cell (ADSC) microspheres and treat neurogenic erectile dysfunction (ED).
Methods: Firstly, an MCSRB was constructed using a centrifugal device and hinged trays. Secondly, influence factors (density, rotational speed) on the formation of ADSC-spheroids were explored. Finally, a neurogenic ED model was established to verify the effectiveness and safety of ADSC-spheroids for ED treatment.
Results: An MCSRB promoted ADSCs to gather microspheres, most of which were 90-130 μm in diameter. Supernatant from three-dimensional culture led to a significant increase in cytokine expression in ADSCs and migration rate in human umbilical vein endothelial cells (HUVECs) compared to control groups. The erectile function and pathological changes of the penis were improved in the ADSC-spheroids treatment group compared to the traditional ADSCs treatment group (p < 0.01).
Conclusion: Efficient, batch, controlled and homogenized production of ADSC stem cell microspheres, and effective improvement of erectile dysfunction in neurogenic rats can be achieved using the MCSRB device.
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Cite this article as:
Wang Peng, Liu Yang, Duan Xiao-Feng, Pan Xiao-Ying, Kong Xiang-Rui and Yang Yong*, A Novel Multi-compartment Rotating Bioreactor for Improving ADSC-Spheroid Formation and its Application in Neurogenic Erectile Dysfunction, Current Stem Cell Research & Therapy 2024; 19 (10) . https://dx.doi.org/10.2174/011574888X253599231126161254
DOI https://dx.doi.org/10.2174/011574888X253599231126161254 |
Print ISSN 1574-888X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3946 |
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