摘要
介绍:巨噬细胞迁移抑制因子(MIF)在类风湿关节炎(RA)的发展中起着关键作用。先前的研究表明,MIF可以触发RA患者和对照组(CS)外周血单核细胞(PBMC)中与Th1、Th2和Th17反应相关的细胞因子谱的表达。尽管如此,迄今为止很少有研究能准确地阐明所涉及的分子机制。本研究旨在探讨CS和RA患者PBMC中Th分化TF (T-bet、GATA-3、RORγt)与MIF受体(CD44、CD74、CXCR2、4,7)和Th1、Th2、Th17细胞因子的表达关系。 方法:两组PBMC培养24 h,流式细胞术检测典型和非典型MIF受体及TF的表达。此外,采用多重头分析来评估培养上清液中细胞因子的水平。研究结果显示,CS组的T CD4+淋巴细胞CD74表达升高(p<0.05),而RA患者的CXCR7表达升高(p<0.001)。此外,与CS组相比,RA患者的T CD4+淋巴细胞表现出更高的GATA3, RORγt和FOXP3表达,以及促炎细胞因子水平升高(p<0.001)。 结果:这些结果表明CD74在CS组的PBMC中表达更显著,而CXCR7在RA患者的PBMC中表达更多。 结论:我们还注意到RA中Th17谱细胞因子的分泌增加,这可能受到CD74和rr γt通过CXCR7通过内噬途径激活FOXP3的影响。
关键词: 类风湿关节炎,MIF, CD74, CD44, CXCR,转录因子,细胞因子。
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