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Research Article

T辅助分化转录因子(T-bet、GATA-3、RORγt和FOXP3)、MIF受体(CD44、CD74、CXCR2、4,7)和Th1、Th2和Th17细胞因子在对照组和类风湿关节炎患者PBMC中的表达

卷 24, 期 9, 2024

发表于: 03 October, 2023

页: [1169 - 1182] 页: 14

弟呕挨: 10.2174/0115665240260976230925095330

价格: $65

摘要

介绍：巨噬细胞迁移抑制因子(MIF)在类风湿关节炎(RA)的发展中起着关键作用。先前的研究表明，MIF可以触发RA患者和对照组(CS)外周血单核细胞(PBMC)中与Th1、Th2和Th17反应相关的细胞因子谱的表达。尽管如此，迄今为止很少有研究能准确地阐明所涉及的分子机制。本研究旨在探讨CS和RA患者PBMC中Th分化TF (T-bet、GATA-3、RORγt)与MIF受体(CD44、CD74、CXCR2、4,7)和Th1、Th2、Th17细胞因子的表达关系。 方法：两组PBMC培养24 h，流式细胞术检测典型和非典型MIF受体及TF的表达。此外，采用多重头分析来评估培养上清液中细胞因子的水平。研究结果显示，CS组的T CD4+淋巴细胞CD74表达升高(p<0.05)，而RA患者的CXCR7表达升高(p<0.001)。此外，与CS组相比，RA患者的T CD4+淋巴细胞表现出更高的GATA3, RORγt和FOXP3表达，以及促炎细胞因子水平升高(p<0.001)。 结果：这些结果表明CD74在CS组的PBMC中表达更显著，而CXCR7在RA患者的PBMC中表达更多。 结论：我们还注意到RA中Th17谱细胞因子的分泌增加，这可能受到CD74和rr γt通过CXCR7通过内噬途径激活FOXP3的影响。

关键词: 类风湿关节炎，MIF, CD74, CD44, CXCR，转录因子，细胞因子。

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