Login Register Cart 0
Generic placeholder image

Current Nutrition & Food Science

Editor-in-Chief

ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Guavira Fruit Pomace Promotes Immunomodulation and Reduction of Tumor Growth in Walker 256 Tumor-Bearing Rats

Author(s): Natália Eirão Zulin, Carolina Garcia Martins, Márcia Helena Appel, Débora Salles da Silva Coutinho, Amanda Plaça Bialli, Daiana Milena Bronoski, Stephanie Rubianne Silva Carvalhal, Elisvania Freitas dos Santos, Paulo Sérgio Loubet Filho, Sandro José Ribeiro Bonatto, Luiz Cláudio Fernandes, Lucimara Mach Côrtes Cordeiro and Fabíola Iagher*

Volume 20, Issue 9, 2024

Published on: 17 October, 2023

Page: [1145 - 1154] Pages: 10

DOI: 10.2174/1573401319666230912105912

Price: $65

Abstract

Background: Guavira fruit is widely used for juice extraction purposes, and this process generates large amounts of pomace (waste). Guavira pomace was dried and milled to produce guavira pomace flour (GPF), which is rich in antioxidants and dietary fibers (polysaccharides). These compounds are known for their immunomodulatory and antitumor effects.

Objective: To investigate whether GPF intake promotes immunomodulation and reduces Walker 256 tumor growth in rats.

Methods: GPF was provided to Wistar rats in two different models: 1) 15-day Model, according to which, Walker 256 tumor-bearing rats received GPF (63 mg/200 g b.w./day) simultaneously to tumor growth for 15 days; 2) 45-day Model, according to which, tumor-bearing rats received GPF for 30 days before tumor cell implantation, as well as during tumor growth - it totaled 45 days. After animals were euthanized, tumors were collected and weighed, and tumor cells were isolated for proliferation capacity determination ex vivo. Enzymatic/colorimetric methods were used to determine resident peritoneal macrophages’ functionality, whereas blood T and B lymphocytes were assayed for proliferation capacity, ex vivo, under stimuli.

Results: The 15-day Model did not show tumor mass or cell proliferation reduction in the treated group. GPF stimulated macrophage response in tumor-bearing and non-tumor-bearing rats. However, there was a substantial reduction in tumor mass and tumor cell proliferation under the 45-day Model. Macrophage and lymphocyte response decreased; it suggested that GPF can directly act in the tumor.

Conclusion: Based on these findings, GPF has immunomodulatory and antitumor actions, and ingestion time plays a key role in them.

Keywords: Campomanesia sp., guavira, fruit pulp industry, Walker tumor, immune response, polysaccharides.

« Previous Next »
Graphical Abstract

[1]
WHO World Health Organization. Updated fact sheet in February 2022. 2022. Available from: https://www.who.int/news-room/fact-sheets/detail/cancer [Accessed July 13, 2023]
[2]
Veronese N, Solmi M, Caruso MG, et al. Dietary fiber and health outcomes: An umbrella review of systematic reviews and meta-analyses. Am J Clin Nutr 2018; 107(3): 436-44.
[http://dx.doi.org/10.1093/ajcn/nqx082] [PMID: 29566200]
[3]
Chikara S, Nagaprashantha LD, Singhal J, Horne D, Awasthi S, Singhal SS. Oxidative stress and dietary phytochemicals: Role in cancer chemoprevention and treatment. Cancer Letters 2017; 413: 122-34.
[http://dx.doi.org/10.1016/j.canlet.2017.11.002]
[4]
AL-Ishaq RK, Overy AJ, Büsselberg D. Phytochemicals and gastrointestinal cancer: Cellular mechanisms and effects to change cancer progression. Biomolecules 2020; 10(1): 105.
[http://dx.doi.org/10.3390/biom10010105] [PMID: 31936288]
[5]
Kumar V, Sinha AK, Makkar HPS, de Boeck G, Becker K. Dietary roles of non-starch polysaccharides in human nutrition: A review. Crit Rev Food Sci Nutr 2012; 52(10): 899-935.
[http://dx.doi.org/10.1080/10408398.2010.512671] [PMID: 22747080]
[6]
Li D, Li J, Dong H, et al. Pectin in biomedical and drug delivery applications: A review. Int J Biol Macromol 2021; 185: 49-65.
[http://dx.doi.org/10.1016/j.ijbiomac.2021.06.088]
[7]
Pedrosa LF, Raz A, Fabi JP. The complex biological effects of pectin: Galectin-3 targeting as potential human health improvement? Biomolecules 2022; 12(2): 289.
[http://dx.doi.org/10.3390/biom12020289] [PMID: 35204790]
[8]
Haminiuk CWI, Maciel GM, Plata-Oviedo MSV, Peralta RM. Phenolic compounds in fruits-an overview. Int J Food Sci Technol 2012; 47(10): 2023-44.
[http://dx.doi.org/10.1111/j.1365-2621.2012.03067.x]
[9]
Schneider VS, Iacomini M, Cordeiro LMC. β-L-Araf-containing arabinan and glucuronoxylan from guavira fruit pomace. Carbohydr Res 2019; 481: 16-22.
[http://dx.doi.org/10.1016/j.carres.2019.06.005] [PMID: 31220627]
[10]
de Paulo Farias D, Neri-Numa IA, de Araújo FF, Pastore GM. A critical review of some fruit trees from the Myrtaceae family as promising sources for food applications with functional claims. Food Chem 2020; 306: 125630.
[http://dx.doi.org/10.1016/j.foodchem.2019.125630] [PMID: 31593892]
[11]
Pascoal A, Ehrenfried C, Lopez B, et al. Antiproliferative activity and induction of apoptosis in PC-3 cells by the chalcone cardamonin from Campomanesia adamantium (Myrtaceae) in a bioactivity-guided study. Molecules 2014; 19(2): 1843-55.
[http://dx.doi.org/10.3390/molecules19021843] [PMID: 24514747]
[12]
Lima e Silva MCB, Bogo D, Alexandrino CAF, et al. Antiproliferative activity of extracts of Campomanesia adamantium (Cambess.) O. berg and isolated compound dimethylchalcone against B16-F10 murine melanoma. J Med Food 2018; 21(10): 1024-34.
[http://dx.doi.org/10.1089/jmf.2018.0001] [PMID: 29715052]
[13]
Viscardi DZ, Oliveira VS, Arrigo JS, et al. Anti-inflammatory, and antinociceptive effects of Campomanesia adamantium microencapsulated pulp. Rev Bras Farmacogn 2017; 27(2): 220-7.
[http://dx.doi.org/10.1016/j.bjp.2016.09.007]
[14]
Schneider VS, Bark JM, Winnischofer SMB, Dos Santos EF, Iacomini M, Cordeiro LMC. Dietary fibres from guavira pomace, a co-product from fruit pulp industry: Characterization and cellular antioxidant activity. Food Res Int 2020; 132: 109065.
[http://dx.doi.org/10.1016/j.foodres.2020.109065] [PMID: 32331640]
[15]
Turin de Oliveira MN, Barbosa da Luz B, Schneider VS, et al. Dietary polysaccharides from guavira pomace, a co-product from the fruit pulp industry, display therapeutic application in gut disorders. Food Res Int 2022; 156: 111291.
[http://dx.doi.org/10.1016/j.foodres.2022.111291] [PMID: 35651057]
[16]
Cazarin CBB, da Silva JK, Colomeu TC, et al. Passiflora edulis peel intake and ulcerative colitis: Approaches for prevention and treatment. Exp Biol Med 2014; 239(5): 542-51.
[http://dx.doi.org/10.1177/1535370214525306] [PMID: 24623393]
[17]
da Silva JK, Cazarin CBB, Batista ÂG, Maróstica M Jr. Effects of passion fruit (Passiflora edulis) byproduct intake in antioxidant status of Wistar rats tissues. Lebensm Wiss Technol 2014; 59(2): 1213-9.
[http://dx.doi.org/10.1016/j.lwt.2014.06.060]
[18]
Martins CG, Appel MH, Coutinho DSS, et al. Consumption of latex from Euphorbia tirucalli L. promotes a reduction of tumor growth and cachexia, and immunomodulation in Walker 256 tumor-bearing rats. J Ethnopharmacol 2020; 255(12): 112722.
[http://dx.doi.org/10.1016/j.jep.2020.112722] [PMID: 32114165]
[19]
Rosen H, Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J Exp Med 1987; 166(6): 1685-701.
[http://dx.doi.org/10.1084/jem.166.6.1685] [PMID: 2445894]
[20]
Belo SRB, Iagher F, Bonatto SJ, et al. Walker-256 tumor growth is inhibited by the independent or associative chronic ingestion of shark liver and fish oil: A response linked by the increment of peritoneal macrophages nitrite production in Wistar rats. Nutr Res 2010; 30(11): 770-6.
[http://dx.doi.org/10.1016/j.nutres.2010.09.015] [PMID: 21130296]
[21]
Bonatto SJR, Folador A, Aikawa J, et al. Lifelong exposure to dietary fish oil alters macrophage responses in Walker 256 tumor-bearing rats. Cell Immunol 2004; 231(1-2): 56-62.
[http://dx.doi.org/10.1016/j.cellimm.2004.12.001] [PMID: 15919370]
[22]
Campos JF, Espindola PPT, Torquato HFV, et al. Leaf and root extracts from Campomanesia adamantium (Myrtaceae) promote apoptotic death of leukemic cells via activation of intracellular calcium and caspase-3. Front Pharmacol 2017; 8(8): 466.
[http://dx.doi.org/10.3389/fphar.2017.00466] [PMID: 28855870]
[23]
Lorençoni MF, Figueira MM, Toledo e Silva MV, et al. Chemical composition and anti-inflammatory activity of essential oil and ethanolic extract of Campomanesia phaea (O. Berg.) Landrum leaves. J Ethnopharmacol 2020; 252: 112562.
[http://dx.doi.org/10.1016/j.jep.2020.112562] [PMID: 31954197]
[24]
Adami ER, Corso CR, Turin-Oliveira NM, et al. Antineoplastic effect of pectic polysaccharides from green sweet pepper (Capsicum annuum) on mammary tumor cells in vivo and in vitro. Carbohydr Polym 2018; 201(201): 280-92.
[http://dx.doi.org/10.1016/j.carbpol.2018.08.071] [PMID: 30241820]
[25]
Stipp MC, Bezerra IL, Corso CR, et al. Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats. Carbohydr Polym 2017; 160(160): 123-33.
[http://dx.doi.org/10.1016/j.carbpol.2016.12.047] [PMID: 28115086]
[26]
Varghese S, Joseph MM, S R A, Unnikrishnan BS, Sreelekha TT. The inhibitory effect of anti- tumor polysaccharide from Punica granatum on metastasis. Int J Biol Macromol 2017; 103: 1000-10.
[http://dx.doi.org/10.1016/j.ijbiomac.2017.05.137] [PMID: 28552725]
[27]
Tamiello CS, Adami ER, de Oliveira NMT, Acco A, Iacomini M, Cordeiro LMC. Structural features of polysaccharides from edible jambo (Syzygium jambos) fruits and antitumor activity of extracted pectins. Int J Biol Macromol 2018; 118(Pt B): 1414-21.
[http://dx.doi.org/10.1016/j.ijbiomac.2018.06.164] [PMID: 30170360]
[28]
Barbat A, Gloaguen V, Moine C, et al. Structural characterization and cytotoxic properties of a 4-O-methylglucuronoxylan from castanea sativa. 2. Evidence of a structure-activity relationship. J Nat Prod 2008; 71(8): 1404-9.
[http://dx.doi.org/10.1021/np800207g] [PMID: 18646856]
[29]
Melo-Silveira RF, Viana RLS, Sabry DA, et al. Antiproliferative xylan from corn cobs induces apoptosis in tumor cells. Carbohydr Polym 2019; 210: 245-53.
[http://dx.doi.org/10.1016/j.carbpol.2019.01.073] [PMID: 30732760]
[30]
Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV. An overview on the role of dietary phenolics for the treatment of cancers. Nutrition Journal 2016; 15(1): 15-99.
[http://dx.doi.org/10.1186/s12937-016-0217-2]
[31]
Leclere L, Cutsem PV, Michiels C. Anti-cancer activities of pH- or heat-modified pectin. Front Pharmacol 2013; 4(128): 128.
[http://dx.doi.org/10.3389/fphar.2013.00128] [PMID: 24115933]
[32]
Vos AP, M’Rabet L, Stahl B, Boehm G, Garssen J. Immune-modulatory effects and potential working mechanisms of orally applied nondigestible carbohydrates. Crit Rev Immunol 2007; 27(2): 97-140.
[http://dx.doi.org/10.1615/CritRevImmunol.v27.i2.10] [PMID: 17725499]
[33]
Wang Y, Zhang N, Kan J, et al. Structural characterization of water-soluble polysaccharide from Arctium lappa and its effects on colitis mice. Carbohydr Polym 2019; 213: 89-99.
[http://dx.doi.org/10.1016/j.carbpol.2019.02.090] [PMID: 30879693]
[34]
Schreiber RD, Old LJ, Smyth MJ. Cancer immunoediting: Integrating immunity’s roles in cancer suppression and promotion. Science 2011; 331(6024): 1565-70.
[http://dx.doi.org/10.1126/science.1203486] [PMID: 21436444]

Rights & Permissions Print Cite
Article Metrics
13
1
Wayfinder Image
© 2024 Bentham Science Publishers | Privacy Policy