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Recent Advances in Anti-Infective Drug Discovery

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ISSN (Print): 2772-4344
ISSN (Online): 2772-4352

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Research Article

Aloe-emodin Quantification using HPTLC and RP-UHPLC in Extracts and Commercial Herbal Formulations: Evaluation of Antimicrobial and Antioxidant Effects

Author(s): Ganesh Dakne, Mohamad Taleuzzaman, Mohammad Sarafroz, Pankaj Yadav, Yasmin Khatoon, Ziyaul Haque and Sufiyan Ahmad*

Volume 18, Issue 3, 2023

Published on: 22 March, 2023

Page: [239 - 253] Pages: 15

DOI: 10.2174/2772434418666230222085606

Price: $65

Abstract

Background: High-performance thin-layer chromatography (HPTLC) was developed and validated for the determination of aloe-emodin in accordance with ICH guidelines. In addition, a novel RP-UHPLC method was developed, and both methods were used to analyse the herbal extract and herbal formulation.

Methods: Separation was carried out on a silica gel 60 F254 HPTLC plate using the mobile phase Toluene: Methanol (9:1). The linearity was good across the 800-4000 ng/spot range. Validation results are within acceptable limits. The percent RSD for accuracy was 0.58-1.77, and precision was 1.10-1.97 and 1.45-1.94 for intraday and interday, respectively. The percentage of aloe-emodin found in the herbal extract and aloe vera capsule was 99.83 ± 1.19 and 99.53 ± 1.29, respectively, using this method.

Results: Quantification of aloe-emodin in herbal extract and herbal formulation were done using a novel UHPLC method with chromatographic conditions of orthophosphoric acid Methanol (0.1 percent OPA): Water (65:35, v/v) and pH 3, a flow rate of 1.2 ml/min, and elute detection at 254 nm. At 6.32 minutes, a sharp and symmetric peak was observed. The method developed was validated in accordance with ICH guidelines. The percent RSD numerical value of accuracy was 0.304-0.576, and the inter-day and intraday precision were 0.32-3.08 and 0.51-2.78, respectively. Herbal extract and aloe vera capsule were analysed using the new UHPLC method. Aloe-emodin percentages were reported as 100.3 ± 0.89 and 99.53 ± 1.29, respectively.

Conclusion: The antimicrobial and anti-oxidant activities of an aloe-vera herbal formulation were studied, and the results were positive.

Keywords: Aloe emodin, HPTLC, UHPLC, aloe vera extract, herbal formulation, antioxidant.

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