Crystalization and preliminary x-ray analysis of thermoactinomyces vulgaris R-47 α-amylase 1

Title:Crystalization and preliminary x-ray analysis of thermoactinomyces vulgaris R-47 α-amylase 1

Volume: 7 Issue: 3

Author(s): Shin Kondo, Akira Kaji, Kazuyuki Yuguchi, Takashi Tonozuka, Yoshiyuki Sakano and Shigehiro Kamitori

Affiliation:

Abstract: Crystals of Thermoactinomyces vulgaris R-47 a-amylase I, hydrolyzing cyclodextrins and pullulan, were grown by the vapor diffusion methods in two crystal forms with minor variations. The diffraction data were collected up to 2.5 A resolution and both crystals were belong to monoclinic system and a space group of C2 with cell dimensions ofa = 123.6 A, b = 51.7 A, and c = 109.1 A , 13 = 104.2° (crystal form 1) and a= 122.5 A, b = 55.0 A, and c = 108.7 A,13 = 103.8° (crystal form 2).

Cite this article as:

Kondo Shin, Kaji Akira, Yuguchi Kazuyuki, Tonozuka Takashi, Sakano Yoshiyuki and Kamitori Shigehiro, Crystalization and preliminary x-ray analysis of thermoactinomyces vulgaris R-47 α-amylase 1, Protein & Peptide Letters 2000; 7 (3) . https://dx.doi.org/10.2174/092986650703221206125753