Single chain anti-idiotypic antibody mimics of the phytotoxin dothistromin

Title:Single chain anti-idiotypic antibody mimics of the phytotoxin dothistromin

Volume: 7 Issue: 3

Author(s): Vadim L. Mett, William T. Jones*, Valentina A. Mett, Dawn Harvey and Paul H.S. Reynolds

Affiliation:

• Plant hnprovement Division, The Horticulture and Food Research Institute of New Zealand, Private Bag 11030, Palmerston North, New Zealand

Abstract: A single chain antibody mimic (MScFvlOE7) was constructed, using a phage display recombinant antibody system, from RNA isolated from the splenocytes of mice immunised with monoclonal antibody MAblOC12 which recognises the furan ring of DOTH [l]. MScFvlOE7 competed with DOTH for binding to MAblOC12 in two ELISA formats and .also reacted with the DOTH binding protein [2] extracted from mature Pinus radiata embryos and is a -type anti-idiotypic mimic of DOTH.

Cite this article as:

Mett L. Vadim, Jones T. William*, Mett A. Valentina, Harvey Dawn and Reynolds H.S. Paul, Single chain anti-idiotypic antibody mimics of the phytotoxin dothistromin, Protein & Peptide Letters 2000; 7 (3) . https://dx.doi.org/10.2174/092986650703221206122640