Abstract
A single chain antibody mimic (MScFvlOE7) was constructed, using a phage display recombinant antibody system, from RNA isolated from the splenocytes of mice immunised with monoclonal antibody MAblOC12 which recognises the furan ring of DOTH [l]. MScFvlOE7 competed with DOTH for binding to MAblOC12 in two ELISA formats and .also reacted with the DOTH binding protein [2] extracted from mature Pinus radiata embryos and is a -type anti-idiotypic mimic of DOTH.
Protein & Peptide Letters
Title:Single chain anti-idiotypic antibody mimics of the phytotoxin dothistromin
Volume: 7 Issue: 3
Author(s): Vadim L. Mett, William T. Jones*, Valentina A. Mett, Dawn Harvey and Paul H.S. Reynolds
Affiliation:
- Plant hnprovement Division, The Horticulture and Food Research Institute of New Zealand, Private Bag 11030, Palmerston North, New Zealand
Abstract: A single chain antibody mimic (MScFvlOE7) was constructed, using a phage display recombinant antibody system, from RNA isolated from the splenocytes of mice immunised with monoclonal antibody MAblOC12 which recognises the furan ring of DOTH [l]. MScFvlOE7 competed with DOTH for binding to MAblOC12 in two ELISA formats and .also reacted with the DOTH binding protein [2] extracted from mature Pinus radiata embryos and is a -type anti-idiotypic mimic of DOTH.
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Cite this article as:
Mett L. Vadim, Jones T. William*, Mett A. Valentina, Harvey Dawn and Reynolds H.S. Paul, Single chain anti-idiotypic antibody mimics of the phytotoxin dothistromin, Protein & Peptide Letters 2000; 7 (3) . https://dx.doi.org/10.2174/092986650703221206122640
DOI https://dx.doi.org/10.2174/092986650703221206122640 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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