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Current Pharmaceutical Biotechnology

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ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

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Research Article

LC-MS/MS Determination of Apigenin in Rat Plasma and Application to Pharmacokinetic Study

Author(s): Shixing Zhu*, Jiayuan Zhang*, Zhihua Lv and Mingming Yu

Volume 22, Issue 2, 2021

Published on: 07 August, 2020

Page: [274 - 280] Pages: 7

DOI: 10.2174/1389201021666200807113144

Price: $65

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Abstract

Background: Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties.

Objective: In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma.

Methods: Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in 2mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model.

Results: Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to 90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500ng/mL. The inter-day and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%.

Conclusion: The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.

Keywords: Apigenin, LC-MS/MS, pharmacokinetic study, rats, validation, plant flavone.

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