![conference banner](/images/conference-banner.jpeg)
Abstract
Background: A large number of Euphorbia species have been evaluated for anticancer effects; however, their anticancer mechanisms have not been established up to now.
Objective: The present study aimed to evaluate the effects of Euphorbia microsciadia (E. microsciadia) Boiss on the modulation of micro (mi) RNAs in MDA-MB-231 cell line.
Methods: As the first step, the inhibitory concentration of hydroalcoholic extract of E. microsciadia on MDA-MB-231 cells was examined using the MTT assay, bypassing 24 and 48h from seeding. The real-time quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) was also utilized to determine Let-7, miR-15, miR-16, miR-29, miR-151, miR-155, miR-21, miR-146b, miR-181b, miR-221, miR-222, miR-21, and miR-146b expressions in MDA-MB-231 cells, by passing 24 and 48h from treating with the extract of E. microsciadia.
Results: The results reveal the cytotoxic effects of E. microsciadia on MDA-MB-231 cell line in a dose-dependent manner. The half maximal Inhibitory Concentrations (IC50) were also equal to 275 and 240μg/ml for E. microsciadia, by passing 24 and 48h from the treatment, respectively. Furthermore, it was confirmed that, E. microsciadia had augmented the expression levels of Let-7, miR-15, miR-16, miR-29, and miR-34a, which lead to an increase in apoptosis.
Conclusion: E. microsciadia could modulate some miRNAs involved in cell cycle arrest and apoptosis in MDA-MB-231 cell line. Accordingly, targeting miRNAs by E. microsciadia can open some newer avenues for breast cancer therapy.
Keywords: Apoptosis, breast cancer, cell cycle, euphorbia, microRNA, miR-34a, tumor suppressor.
[http://dx.doi.org/10.3390/ijms160510888] [PMID: 25984608]
[http://dx.doi.org/10.2174/1574892814666190111104834] [PMID: 30636616]
[http://dx.doi.org/10.2174/1574892813666181119123035] [PMID: 30451116]
[http://dx.doi.org/10.22037/ijpr.2010.910] [PMID: 24381609]
[http://dx.doi.org/10.5812/jjnpp.63640]
[PMID: 28630384]
[http://dx.doi.org/10.1080/15376516.2017.1347735] [PMID: 28651465]
[http://dx.doi.org/10.1042/BSR20181538] [PMID: 30459240]
[http://dx.doi.org/10.1080/14786419.2014.979418] [PMID: 25426544]
[http://dx.doi.org/10.1016/j.jep.2017.08.014] [PMID: 28811220]
[http://dx.doi.org/10.1016/j.btre.2019.e00376] [PMID: 31641620]
[http://dx.doi.org/10.1007/s10637-019-00728-0] [PMID: 30706338]
[http://dx.doi.org/10.1080/14786419.2017.1305380] [PMID: 28403658]
[http://dx.doi.org/10.4103/2221-1691.275419]
[http://dx.doi.org/10.1016/j.phymed.2020.153168] [PMID: 31982837]
[http://dx.doi.org/10.1134/S0006297918090031] [PMID: 30472940]
[http://dx.doi.org/10.5530/ijper.51.3s.71]
[http://dx.doi.org/10.4103/jrms.JRMS_967_16] [PMID: 29387117]
[http://dx.doi.org/10.1055/s-0042-108450] [PMID: 27272400]
[http://dx.doi.org/10.2174/157489211793980033]
[http://dx.doi.org/10.2174/1574892810666150120163617] [PMID: 25600282]
[http://dx.doi.org/10.2174/157489010793351962] [PMID: 20649511]
[http://dx.doi.org/10.2174/1574892809666140307101519] [PMID: 24605908]
[http://dx.doi.org/10.2174/157489212798357958] [PMID: 21854360]
[http://dx.doi.org/10.1007/s11095-010-0105-y] [PMID: 20306121]
[http://dx.doi.org/10.1631/jzus.B1400184] [PMID: 25559952]
[PMID: 21311629]
[http://dx.doi.org/10.1371/journal.pone.0191195] [PMID: 29324832]
[http://dx.doi.org/10.1038/cddis.2016.6] [PMID: 26890143]
[http://dx.doi.org/10.2174/13894501113149990189] [PMID: 23834152]
[http://dx.doi.org/10.1051/bmdcn/2018080208] [PMID: 29806586]
[http://dx.doi.org/10.4103/1947-2714.104316] [PMID: 23272307]
[http://dx.doi.org/10.1016/j.cell.2007.10.054] [PMID: 18083101]
[http://dx.doi.org/10.3892/ijo.2013.2034] [PMID: 23900351]
[http://dx.doi.org/10.1093/nar/gkn522] [PMID: 18701644]
[http://dx.doi.org/10.1186/1756-9966-32-98] [PMID: 24289849]
[http://dx.doi.org/10.1371/journal.pone.0168171] [PMID: 27930738]
[http://dx.doi.org/10.1158/0008-5472.CAN-09-4250] [PMID: 20354188]
[http://dx.doi.org/10.1053/j.gastro.2007.05.022] [PMID: 17681183]
[http://dx.doi.org/10.1074/jbc.M804612200] [PMID: 18708351]
[http://dx.doi.org/10.1172/JCI61495] [PMID: 22622040]
[http://dx.doi.org/10.2353/ajpath.2010.090664] [PMID: 20075198]
[http://dx.doi.org/10.1038/onc.2008.171] [PMID: 18504431]
[http://dx.doi.org/10.2174/1389557515666150709115355] [PMID: 26156420]
[http://dx.doi.org/10.1007/s00412-009-0210-z] [PMID: 19340450]
[http://dx.doi.org/10.1073/pnas.0506654102] [PMID: 16166262]
[http://dx.doi.org/10.1007/s12032-009-9344-3] [PMID: 19908170]
[http://dx.doi.org/10.1073/pnas.0800121105] [PMID: 18362358]
[http://dx.doi.org/10.1158/0008-5472.CAN-07-1484] [PMID: 18006822]
[http://dx.doi.org/10.1373/clinchem.2010.151845] [PMID: 21036945]
[http://dx.doi.org/10.3892/br.2016.747] [PMID: 27699004]
[http://dx.doi.org/10.1158/0008-5472.CAN-08-3559] [PMID: 19190326]
[http://dx.doi.org/10.3109/07357907.2010.512596] [PMID: 20874002]
[http://dx.doi.org/10.3892/br.2013.199] [PMID: 24649060]
[http://dx.doi.org/10.1016/j.molonc.2014.01.005] [PMID: 24484937]
[http://dx.doi.org/10.1007/s12288-016-0694-7] [PMID: 28824233]
[http://dx.doi.org/10.3892/ijo.2013.2059] [PMID: 23939688]
[http://dx.doi.org/10.1038/onc.2010.487] [PMID: 21057537]
[http://dx.doi.org/10.1080/21691401.2019.1637886] [PMID: 31343368]
[http://dx.doi.org/10.1016/j.ejphar.2019.03.005] [PMID: 30851271]
[http://dx.doi.org/10.1158/0008-5472.CAN-07-1585] [PMID: 17823410]
[http://dx.doi.org/10.1038/srep46186] [PMID: 28383524]
[http://dx.doi.org/10.18632/oncotarget.1825] [PMID: 24657911]
[http://dx.doi.org/10.1111/cas.12656] [PMID: 25783790]
[http://dx.doi.org/10.1007/s11010-013-1723-6] [PMID: 23771315]
[http://dx.doi.org/10.1016/j.brainres.2010.07.009] [PMID: 20633539]
[http://dx.doi.org/10.1007/s00125-017-4237-z] [PMID: 28280903]
[http://dx.doi.org/10.7868/S0026898416010043] [PMID: 27028817]
[http://dx.doi.org/10.1002/ptr.5878] [PMID: 28752532]
![Wayfinder Image](/images/wayfinder.jpg)