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摘要
背景:已经合成并通过NMR光谱仪鉴定并合成了6-羟基黄嘌呤二烯的eremophilane系列的倍半萜内酯与氢化杂志(哌啶和哌嗪)的共轭物。 目的:从植物土木香(Inula helenium L)中提取具有异常骨架“ 6-羟基黄嘌呤二烯”的内酯,并用NMR光谱仪鉴定各种物种。 方法:研究了A549,HCT116,RD和Jurkat等不同肿瘤系的细胞毒性,线粒体和抗氧化活性,并确定了可能的机制。 结果:结果表明,最有效的化合物是IIIi,对RD细胞具有最高的细胞毒性(IC50 25.23} 0.04μM),使线粒体膜去极化,并且是一种有效的抗氧化剂(IC50抑制LP 10.68} 3.21μM),没有任何对健康细胞的毒副作用。 结论:倍半萜烯内酯6-羟基黄嘌呤二烯III与氢化叠氮的结合可能有助于设计潜在的有前途的抗癌药物。
关键词: 6-羟基黄嘌呤二烯,叠氮,细胞毒性,线粒体,细胞凋亡,抗氧化剂,抗癌药。
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