摘要
背景:冻伤导致严重的骨骼肌损伤。由于冻伤引起的炎症反应会引起局部肌肉变性。先前的研究表明,热激蛋白(hsps)可以防止发炎。另外,我们以前的研究表明,hsp70的表达增加能够保护骨骼肌免受冰冻作用。 方法:因此,我们的目的是确定热激蛋白的诱导是否能够最大程度地减少炎症并保护骨骼肌免受冻伤。 结果:在本研究中,我们使用了hsp90抑制剂17-二甲基氨基乙基氨基-17-去甲氧基格尔德霉素(17-DMAG),将其在冻伤后30分钟内给药。与对照组大鼠后肢肌肉相比,冻疮伤后注射17-DMAG的大鼠后肢肌肉表现出较少的炎性细胞浸润。与该观察结果一致,已经观察到hsp表达增加导致炎性细胞因子表达减少。此外,我们发现冻伤后给予17-DMAG可以保留肌肉组织结构和功能。 结论:已经得出结论,如果在冻伤损伤后30分钟内注射,诱导热激蛋白的化合物(例如17-DMAG)能够保留骨骼肌的功能和结构。我们的研究为开发治疗冻伤造成的潜在治疗策略提供了基础。
关键词: 冻伤,热休克蛋白,hsp90抑制剂,17-DMAG,骨骼肌,炎症。
Current Molecular Medicine
Title:Increased Heat Shock Protein Expression Decreases Inflammation in Skeletal Muscle During and after Frostbite Injury
Volume: 20 Issue: 9
关键词: 冻伤,热休克蛋白,hsp90抑制剂,17-DMAG,骨骼肌,炎症。
摘要:
Background: Frostbite injury results in serious skeletal muscle damage. The inflammatory response due to frostbite causes local muscle degeneration. Previous studies have shown that heat shock proteins (hsps) can protect against inflammation. In addition, our previous studies showed that increased expression of hsp70 is able to protect skeletal muscle against cryolesion.
Methods: Therefore, our aim was to determine if the induction of the heat shock proteins are able to minimize inflammation and protect skeletal muscle against frostbite injury.
Results: In the present study, we used the hsp90 inhibitor, 17-dimethylaminoethylamino- 17-demethoxygeldanamycin (17-DMAG), which was administered within 30 minutes following frostbite injury. Rat hind-limb muscles injected with 17-DMAG following frostbite injury exhibited less inflammatory cell infiltration as compared to control rat hind-limb muscles. In agreement with this observation, it has been observed that increased hsp expression resulted in decreased inflammatory cytokine expression. Additionally, we found that the administration of 17-DMAG after frostbite injury can preserve muscle tissue structure as well as function.
Conclusion: It has been concluded that compounds such as 17-DMAG that induce the heat shock proteins are able to preserve skeletal muscle function and structure if injected within 30 minutes after frostbite injury. Our studies provide the basis for the development of a potential therapeutic strategy to treat the injury caused by frostbite.
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Cite this article as:
Increased Heat Shock Protein Expression Decreases Inflammation in Skeletal Muscle During and after Frostbite Injury, Current Molecular Medicine 2020; 20 (9) . https://dx.doi.org/10.2174/1566524020666200407083131
DOI https://dx.doi.org/10.2174/1566524020666200407083131 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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