Affiliation: School of Chemistry&Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
The interactions between bovine serum albumin and phenols have been studied by cyclic voltammetry (CV). The interaction divides into specific and non-specific binding sites, and mainly ascribes to the electronic force. One specific binding site between bovine serum albumin and p-aminophenol (p-AP) as well as p-quinonimine (p-AQ) is obtained. Both the specific binding number of p-AP and p-AQ binding bovine serum albumin were in the ratio of 5:1. Two specific binding sites for p-hydroquinone (p-HQ) and p-benzoquinone (p-BQ) were found, respectively. The specific binding number of p-HQ are 15:1 and 9:1, respectively, while total specific binding number was 26:1. Moreover, the specific binding number of p-BQ was 15:1 and 9:1, respectively, with total specific binding number was 26:1. Three specific binding sites for pyrocatechol (PC) were obtained, and two specific binding sites for o-benzoquinone (o-BQ) were observed. The specific binding number of PC are 11:1, 3:1 and 8:1, respectively, total specific binding number being 22:1. However, the specific binding number of o-BQ was 3:1 and 5:1 respectively, with total specific binding number being 8:1.