<![CDATA[Meningoencephalitis]]> https://benthamscience.com RSS Feed for Disease Wise Article | BenthamScience EurekaSelect (+http://eurekaselect.com) Fri, 29 Mar 2024 13:28:24 +0000 <![CDATA[Meningoencephalitis]]> https://benthamscience.com https://benthamscience.com <![CDATA[Emerging Viral Diseases and Potential Therapeutic Agents for Their Control]]>https://benthamscience.comchapter/13247 <![CDATA[Amyloid Hypothesis in Alzheimer´s Disease]]>https://benthamscience.comchapter/12920

So, the question is, what´s wrong about this hypothesis and the immunotherapy approach? These compounds aimed at reducing Aβ formation and plaques do not restore cognition although removes amyloid plaques in PET amyloid scans.

This paper tries to discuss all the aspects and describe the current situation and the future goals.]]> <![CDATA[Sexually Transmitted Co-infections in Persons Living with HIV]]>https://benthamscience.comchapter/12309 <![CDATA[TUBERCULOSIS: Epidemiological, Molecular, Immunological and Clinical Characteristics]]>https://benthamscience.comchapter/11865 <![CDATA[Systemic Fungal Infection]]>https://benthamscience.comchapter/11855 <![CDATA[Neurodegenerative and Neuropsychiatric Disorders: Present Clinical Drug Research and Future Perspectives]]>https://benthamscience.comchapter/11770 <![CDATA[Prevention and Eradication of Biofilm in Medical Indwelling Devices]]>https://benthamscience.comchapter/11121 <![CDATA[Nanomedicine and Neurodegenerative Diseases: An Introduction to Pathology and Drug Targets]]>https://benthamscience.comchapter/10705 <![CDATA[Iatrogenic Lesions in Neurology]]>https://benthamscience.comchapter/10559 <![CDATA[Subject Index]]>https://benthamscience.comchapter/10318 <![CDATA[Free-living Amoebae]]>https://benthamscience.comchapter/10315

Naegleria Fowleri: The organism was first reported in Australia in 1965. It is an environmental ameboflagellate parasite found in variety of water bodies such as ponds, swimming pools; aquarium etc. prefers temperature of 30-45ºC. There are three stages seen in Naegleria life cycle: the infective trophozoites, transient flagellated and the resistant cystic stage. The portal of entity of trophozoites is via olfactory neuroepithelial cell lining covering the cribriform plate to reach olfactory bulb. Demyelination and myelinoclasis are observed in gray matter due to vascular blockage. These pathological changes are attributed to release of phospholipolytic enzymes which causes breaks in the lipid membrane of neuronal cells. Clinically, patients of primary amoebic meningoencephalitis (PAM) usually presented with high grade fever,headache, photophobia and features of raised intracranial pressure. Laboratory diagnosis is done using peripheral smear, CSF examination, culture, histopathological examination and imaging modalities. Hematological findings are leukocytosis with predominant neutrophils. The CSF shows low glucose and high protein levels. Centrifugation of fresh CSF sample up to 500 RPM may reveal motile trophozoites. Morphologically, the size of trophozoites ranges between 12-25μm, with a single nucleus and centrally placed nucleolus in the absence of peripheral chromatin. Liquid culture media such as such as Nelson’s medium containing ox liver digest and glucose are used with serum for growing amoebae. Mammalian cell lines can be employed to demonstrate cytopathic effect. Multiplex PCR detects free living amoeba within 6 hours but routine use in diagnostic laboratory is limited due to rarity of finding these organisms and having high cost of PCR. Brain imaging is easy to perform but restricted by nonspecific findings such as cerebral edema. Specifically, infraction involving frontal, orbital and cerebellum area can be observed in few cases of PAM. There is no optimal treatment regime for Naegleria fowleri. Literature suggests combination therapy works best with amphotericin, rifampicin and azithromycin.

Balamuthia Mandrillaris: Over 200 cases were reported from South America and United States. The true prevalence of disease is unknown in south East Asia. Organism is commonly isolated from soil contact with activities related to soil such as gardening, agriculture pose risk of acquiring the organism. It was first isolated in 1986 from baboon brain that died of meningoencephalitis. The portal of entry of the organism is via cutaneous lesions, nasal mucosa and then subsequent spread to brain. CNS lesions mimic acanthamoeba encephalitis and have chronic slowly progressive course over many years. The life cycle of Balamuthia involves two stages: trophozoites and the cyst. The morphologically variable trophozoites are 12- 60 microns in size containing single nucleus with large centrally placed nucleolus. Cysts are spherical in shape measuring 12-30μm and contain a single nucleus with double wall having outer ectocyst, middle fibrillar layer and inner amorphous endocyst.The trophozoites, cysts and inflammatory cells are observed in perivascular regions of the infected tissue. In CSF, elevated protiens, reduced glucose are common findings. Balamuthia spp. can be grown in tissue cultures such as Monkey Kidney cell lines, Human Lung fibroblast and Human Brain Microvascular Endothelial cell lines. ELISA test is very specific to detect high antibodies titers. The antibodies do not cross react with other free living amoebae. PCR is also highly specific and sensitive test in which primers are developed against mitochondrial rRNA genes. Recently, real time PCR are developed targeting RNAase P gene of B.mandrillaris.

Sappinia Species: Two species of Sappinia are well-known cause of CNS infections in humans, Sappinia diplodea and Sappinia pedata. S.diploidea was first isolated from lizard faeces. As this parasite is found in animal faeces, persons handing livestock are at higher risk. Only one known case of Sappinia encephalitis infection reported in literature. The diagnosis was confirmed on histopathological sample, which showed necrotizing haemorrhagic inflammation of infected tissue, containing trophozoites. The trophozoite of Sappinia is characterized by two opposing nucleus with central flattening. Diagnosis can also be done by amplifying rDNA of both Sappinia diploidea and Sappinia pedata using SSU primers. The real time PCR can also be used based on 18rRNA gene sequences. Sappinia spp. is cultivated on non- nutrient agar with overlay of Enterobacter or Escherichia coli. Vahlkamphia spp. and Paravahlkamfia francinae are other emerging free living amoebas that were first isolated from CSF of young patient who presented with typical symptoms of primary amoebic meningoencephalitis.]]> <![CDATA[Acute Posterior Multifocal Placoid Pigment Epitheliopathy]]>https://benthamscience.comchapter/10223