Contents

Combinatorial Chemistry & High Throughput Screening, Volume 6 - Number 4

Preface (Hot Topic: Fluorescence-based Screening Methods Guest Editor: Kyle R. Gee ).

, 6(4): i

Kyle R. Gee


DOI: 10.2174/138620703106298428




Phenotypic Screening of Small Molecule Libraries by High Throughput Cell Imaging

, 6(4): 279 - 286

J. C. Yarrow, Y. Feng, Z. E. Perlman, T. Kirchhausen and T. J. Mitchison


DOI: 10.2174/138620703106298527




A Fluorescent High-Throughput Assay for Double-Stranded DNA: the RediPlate PicoGreen Assay

, 6(4): 287 - 291

Robert Batchelor, David Hagen, Iain Johnson and Joseph Beechem


DOI: 10.2174/138620703106298536




Rapid Assays for Quantitating Cytokine Gene Expression Without Target Amplification

, 6(4): 293 - 301

Frank R. Gonzales, Augusto Lois, Toddy Sewell, Carl LaCerte, Barbara Gaynor, Edison Garcia, Melinda Jazdzewski, Tammy Oliver, Irene Postor and Deirdre Trainor


DOI: 10.2174/138620703106298545




Assay Development and High-Throughput Screening of Caspases in Microfluidic Format

, 6(4): 303 - 312

Ge Wu, Jennifer Irvine, Chris Luft, David Pressley, C. Nicholas Hodge and Bill Janzen


DOI: 10.2174/138620703106298554




Fluorescence Assays for High-Throughput Screening of Protein Kinases

, 6(4): 313 - 320

G. J.R. Zaman, A. Garritsen, Th. de Boer and C. A.A. van Boeckel


DOI: 10.2174/138620703106298563




Competitive Fluorescence Polarization Assays for the Detection of Phosphoinositide Kinase and Phosphatase Activity

, 6(4): 321 - 330

Beth E. Drees, Amber Weipert, Heather Hudson, Colin G. Ferguson, Leena Chakravarty and Glenn D. Prestwich


DOI: 10.2174/138620703106298572




Strategies and Solid-Phase Formats for the Analysis of Protein and Peptide Phosphorylation Employing A Novel Fluorescent Phosphorylation Sensor Dye

, 6(4): 331 - 339

Karen Martin, Thomas H. Steinberg, Terrie Goodman, Birte Schulenberg, Jason A. Kilgore, Kyle R. Gee, Joseph M. Beechem and Wayne F. Patton


DOI: 10.2174/138620703106298581




Development of Fluorescence-Based Selective Assays for Serine / Threonine and Tyrosine Phosphatases

, 6(4): 341 - 346

Christina Pastula, Iain Johnson, Joseph M. Beechem and Wayne F. Patton


DOI: 10.2174/138620703106298590




Homogeneous Fluorescence Assay for Cyclic AMP

, 6(4): 347 - 354

Ewa Heyduk, Ying Fei and Tomasz Heyduk


DOI: 10.2174/138620703106298437




Measuring Intracellular Calcium Fluxes in High Throughput Mode

, 6(4): 355 - 362

Chris Chambers, Fiona Smith, Christine Williams, Sandra Marcos, Zhen Han Liu, Paul Hayter, Giuseppe Ciaramella, Wilma Keighley, Phil Gribbon and Andreas Sewing


DOI: 10.2174/138620703106298446




New Ca2+ Fluoroionophores Based on the BODIPY Fluorophore

, 6(4): 363 - 366

Kyle R. Gee, Aleksey Rukavishnikov and Anca Rothe


DOI: 10.2174/138620703106298455




High Throughput Fluorescence-Based Assays for Cyclic ADP-Ribose, NAADP, and Their Metabolic Enzymes

, 6(4): 367 - 379

Richard M. Graeff and Hon Cheung Lee


DOI: 10.2174/138620703106298464




β Galactosidase Enzyme Fragment Complementation as A Novel Technology for High Throughput Screening

, 6(4): 381 - 387

Richard M. Eglen and Rajendra Singh


DOI: 10.2174/138620703106298473




High Throughput Screening of G-Protein Coupled Receptors via Flow Cytometry

, 6(4): 389 - 397

A. Waller, P. Simons, E. R. Prossnitz, B. S. Edwards and L. A. Sklar


DOI: 10.2174/138620703106298482




Established and Emerging Fluorescence-Based Assays for G-Protein Function: Heterotrimeric G-Protein Alpha Subunits and Regulator of G-Protein Signaling (RGS) Proteins

, 6(4): 399 - 407

Randall J. Kimple, Miller B. Jones, Adam Shutes, Benjamin R. Yerxa, David P. Siderovski and Francis S. Willard


DOI: 10.2174/138620703106298491




Established and Emerging Fluorescence-Based Assays for G-Protein Function: Ras-Superfamily GTPases

, 6(4): 409 - 418

Rafael J. Rojas, Randall J. Kimple, Kent L. Rossman, David P. Siderovski and John Sondek


DOI: 10.2174/138620703106298509




Meet the Guest Editor

, 6(4): 419

Kyle R. Gee


DOI: 10.2174/138620703106298518




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