Practical Biochemistry

Commonly Used Basic and Advanced Techniques

Author(s): Anshika Sah, Sinchan Hait and Sai Joshi *

Pp: 267-287 (21)

DOI: 10.2174/9789815165852124010020

* (Excluding Mailing and Handling)

Abstract

Different techniques are used in a biochemical laboratory to measure various parameters. For example, a pH meter is used to measure the pH of the sample and electrochemical cells are used to measure the redox potential. Zeta potential is another property which can be measured using zeta cells. Chromatography is a technique used to separate the components of a mixture. Two of the most common chromatography techniques are HPLC and ion exchange chromatography. HPLC is based on the interactions of the analytes in stationary as well as mobile phase. Whereas ion exchange chromatography is based on the interaction of ions and charged site of the stationary phase. 


Keywords: Electrochemical cell, Electron transfer, HPLC, Ion exchange, pH, pH meter, Redox, UV detection, Zeta potential, Zeta cell.

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