Title:The Capability to Forecast Response to Therapy with Regard to The Time and Intensity of The Inflammatory Process in vitro in Dermal Fibroblasts Induced by IL-12
Volume: 19
Issue: 15
Author(s): Beniamin Grabarek, Dominika Wcisło-Dziadecka*, Barbara Strzałka-Mrozik, Jolanta Adamska, Urszula Mazurek and Ligia Brzezińska-Wcisło
Affiliation:
- Department of Cosmetology, School of Pharmacy with Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Kasztanowa 3, 41-200 Sosnowiec,Poland
Keywords:
Inflammatory process, IL-12/23 signaling pathways, IFN-γ, TNF-α, fibroblasts, vicious circle, RTqPCR, genes ontology.
Abstract: Background: The aim of the study was to evaluate the changes in the expression of genes -
TNF-α, IFN-γ, depending on the time and concentration of IL-12 used to expose the Normal Human
Dermal Fibroblast (NHDF) cells.
Methods: The material for the study included NHDF exposed to IL-12 in various IL-12 concentrations
(1/10/100 ng/ml) and exposure time 0.5h, 1h, 2h, 4h, 8h, 24h, compared to the control. Changes in
gene expression were evaluated with the use of the RTqPCR method. The statistical analysis was performed
with the use of Statistica 12.5 PL The role of genes of the JAK-STAT signaling pathway in the
induction of the inflammatory process was determined with the use of the PANTHER overrepresentation
test.
Results: Regardless of the time of NHDF exposure and the IL-12 concentrations used, we observed
changes in the expressions of TNF-α and IFN-γ. Increased expression of one transcript involves the
decreased expression of the other. We assessed that genes of the JAK-STAT signaling pathway are engaged
in 6 biological processes, 8 molecular functions, 6 signaling pathways.
Conclusion: The conducted studies indicate that conclusions about the intensity of the inflammatory
process, and the efficacy of the anti-cytokine therapeutic strategy, may be made on the basis of the
TNF-α, IFN-γ expression.
