Title:Synthesis, Biological Evaluation and Docking Studies of 1,3,4-Oxadiazole Fused Benzothiazole Derivatives for Anticancer Drugs
Volume: 15
Issue: 12
Author(s): Madala Subramanyam, Reddymasu Sreenivasulu, Rambabu Gundla, Mandava V.B. Rao and Koya Prabhakara Rao*
Affiliation:
- New Generation Materials Lab (NGML), Department of Science and Humanities, Vignan's Foundation for Science Technology and Research VFSTR (A deemed to be University), Vadlamudi, Guntur-522 213, Andhra Pradesh,India
Keywords:
Anticancer activity, 2-aminobenzothiazole, antitumor agents, 1, 3, 4-oxadiazole, docking studies, cytotoxicity.
Abstract: Background: Hetero atom containing compounds are well studied class of organic compounds
exhibits variety of properties and applications. Design and synthesis of new heterocyclic
compounds are always of great interest in synthetic and medicinal organic chemistry. Benzothiazole
or 2-aminobenzothiazole scaffold based derivatives were reported to display a wide range of
biological activities including anticancer, anti-tubercular, antiviral, fungicidal, etc. On the other
hand, 1,3,4-oxadiazoles were permit to increase their biological activities due to H-bonding with
receptors. These derivatives possess diverse biological activities which include anticancer, antiviral,
antifungal, antibacterial and antidepressant etc. Due to interesting biological activity information of
about these hetero cyclic moieties, benzothiazole/2-aminobenzothiazole and 1,3,4-oxadiazoles
moieties, we chose to design a new series of heterocyclic compounds by mimicking these two types
of scaffolds in a single molecule for our study.
Methods: The 1,3,4-oxadiazole linked benzothiazole derivatives were synthesized by condensation
of, 2-(4-(5-(benzo[d]thiazol-2-yl)-1,3,4-thiadiazol-2-yl)-2,6-dimethoxyphenoxy)acetohydrazide and
POCl3 under reflux conditions. All these ten compounds structures were confirmed by spectral data
1H & 13C NMR, Mass, CHN analysis etc. Further, these compounds were evaluated for their anticancer
activity against four human cancer cell lines, A549, MCF7, A375 and HT-29 in comparison
to CA4 as a reference drug. We also carried out docking studies of these compounds in the Colchicines
binding site of Tubulin (PDB_ID: 1SA0) using Glide docking tool indicated that the ligands
show good interactions with active site residues.
Results: A new series of 1,3,4-oxadiazole fused benzothiazole derivatives were synthesized successfully
in totally six steps starting with 4-hydroxy-3,5-dimethoxybenzoyl chloride. All these
newly synthesized compounds structures were confirmed by spectral studies and elemental analysis.
As we designed for anticancer activity, they were assessed for their anticancer activity against four
human cancer cell lines in comparison to a reference drug CA4. As expected, all the ten compounds
exhibited anticancer activities against four cancer cell lines with half maximal inhibitory concentration
(IC50) values ranging from 0.01 µM to 12.3 µM. The docking studies indicated all the compounds
exhibited good binding energies with the receptor.
Conclusion: In this study we designed a new series heterocyclic compounds by mimicking two
types of scaffolds benzothiazole/2-aminobenzothiazole and 1,3,4-oxadiazoles moieties in a single
molecule based on their biological activity in the literature. They were synthesized successfully and
molecular structures were confirmed by spectral studies. As expected, all the compounds exhibited
anticancer activities against four cancer cell lines. This study can provide a roadmap for design and
synthesis of new drug molecules for antitumor and anticancer activity.
