Title:Re-SILAC: A Simple and Convenient Approach for the Preparation of the SILAC Internal Standards
Volume: 13
Issue: 3
Author(s): Tianying Yu, Tailong Yi, Miaomiao Tian, Zhongwei Xu, Yao Zhang, Linhui Zhai, Xiaoming Yang and Ping Xu
Affiliation:
Keywords:
CCK-8, liquid storage, MHCC97-L, quantitative proteomics, Re-SILAC, SILAC.
Abstract: SILAC (Stable Isotope Labeling with Amino acids in Cell culture) is one of the most
popular methods in quantitative proteomics field. The internal standards of SILAC can mark a variety
of clinical samples or tissues instead of labeling the whole organism and play an important role in
proteomics. Preparing the SILAC internal standards has been difficult and time-consuming for culturing
cell lines, therefore a simple and convenient Re-SILAC method was introduced. Cell lines labeled
with SILAC were thawed in SILAC medium to be remarked again. Comparing the morphology,
size, growth curve and proteome of MHCC97-L between Re-SILAC and SILAC cell lines, it
was found that SILAC-labeled cells that were frozen with liquid nitrogen had no influence on Re-
SILAC-labeled. In conclusion, Re-SILAC method is a useful method for preparing SILAC internal
standards.