Title:Pharmacokinetics and Tissue Distribution of Fargesin After Oral Administration in Rats by High Performance Liquid Chromatography
Volume: 12
Issue: 4
Author(s): Zhi-wu Zhang, Xiao-xiao Liu, Wei Cao, Xiong Xiao, Jun-ge Qu, Ming-gao Zhao and Xiao-qiang Li
Affiliation:
Keywords:
Double-peak, Fargesin, HPLC, oral administration, pharmacokinetics, tissue distribution.
Abstract: Background: Flos Magnoliae is a frequently-used Chinese herbal medicine, from which Fargesin
is isolated with extensive pharmacological activities.
Objective: To develop a reliable high performance liquid chromatography (HPLC) method of fargesin
and explore pharmacokinetics and tissue distribution profile of fargesin in Sprague Dawley rats after oral
administration of 50 mg/kg.
Methods and Results: The chromatographic analysis was conducted on a Shimadzu 2010-ODS-3 C18
column (4.6 mmx150 mm, 5 µm), and the mobile phase consisted of methanol and water (58:42 v/v) with
a flow rate of 1.0 mL/min. Linearity of fargesin in all biological samples was good (R>0.9990) within the
corresponding concentration range and the method was verified to be sensitive, accuracy and precision.
The pharmacokinetic results of fargesin after oral administration (50 mg/kg) in rats showed that two absorption
peaks were observed in rat plasma at 60min and 290 min, with the highest plasma concentration
(Cmax) being 464.38±32.75 ng/mL at 290 min. The tissue distribution results showed that the main tissue
depots for fargesin were heart, liver, kidney and lung.
Conclusion: This study established a sensitive and reliable HPLC method for the content determinations
of fargesin. The pharmacokinetics and tissue distribution results may provide practical information for
further study of pharmacological actions and clinical application.
