Title:Genomic Face-Off: An In Silico Comparison of the Probiotic Potential of Lactobacillus spp. and Akkermansia muciniphila
Volume: 25
Author(s): Nayeema Bulbul, Jinath Sultana, Ashrafus Safa, Md. Asaduzzaman Shishir, Bakhtiar Ul Islam, Md. Fakruddin*Md. Abu Bakar karim
Affiliation:
- Department of Biochemistry & Microbiology, North South University, Bangladesh
Keywords:
In silico, genome, sequence, probiotic, Akkermansia muciniphila, Lactobacillus.
Abstract:
Introduction: The gut microbiota plays a crucial role in maintaining human health, and
probiotics have gained significant attention for their potential benefits. Among the diverse array of
gut bacteria, Akkermansia muciniphila, and Lactobacillus spp. have emerged as promising candidates
for their putative probiotic properties.
Method: In this study, we conducted a comprehensive comparative in silico analysis of the
genomes of A. muciniphila and Lactobacillus to decipher their probiotic potential. Utilizing a
range of bioinformatics tools, we evaluated various genomic attributes, including functional gene
content, metabolic pathways, antimicrobial peptide production, adhesion factors, and stress response
elements. These findings revealed distinctive genomic signatures between the two genera.
A. muciniphila genomes exhibited a high prevalence of mucin-degrading enzymes, suggesting a
specialized adaptation for mucin utilization in the gut environment.
Results: Additionally, the presence of specific pathways for short-chain fatty acid production highlighted
its potential impact on host health. Lactobacillus genomes, on the other hand, demonstrated
a diverse repertoire of functional genes associated with probiotic attributes, including the production
of antimicrobial peptides and adhesion factors, indicating potential for host-microbe interactions
and immune modulation. Furthermore, this analysis unveiled the genetic basis of stress tolerance
in both genera, revealing conserved mechanisms for surviving the dynamic conditions of
the gut ecosystem.
Conclusion: This study also shed light on the distribution of antibiotic-resistance genes, allowing
us to assess safety concerns associated with their potential use as probiotics. Overall, this comparative
in silico exploration provides valuable insights into the genomic foundation of A. muciniphila
and Lactobacillus probiotic potential. These findings contribute to the understanding of their respective
roles within the gut microbiota and offer a foundation for further experimental investigations.
As probiotic applications continue to expand, this study advances our knowledge of the genetic
underpinnings that govern their functionality and highlights promising avenues for future
therapeutic interventions and personalized health strategies.
