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Abstract
Background: Lung cancer is one of the more common malignant tumors posing a great threat to human life, and it is very urgent to find safe and effective therapeutic drugs. The antitumor effect of ginsenosides has been reported to be a treatment with a strong effect and a high safety profile.
Objective: This paper aimed to investigate the inhibitory effect of ginsenoside Rb1 on 95D and NCI-H460 lung cancer cells and its pathway to promote apoptosis.
Methods: We performed the CCK-8 assay, fluorescence staining assay, flow cytometry, scratch healing assay, and Transwell assay to detect the effects of different concentrations of ginsenoside Rb1 on the antitumor activity of 95D and NCI-H460 cells and Western Blot detected the mechanism of antitumor effect.
Results: Ginsenoside Rb1 treatment significantly increased the inhibition and apoptosis rates of 95D and NCIH460 cells and inhibited the cell cycle transition from S phase to G2/M. Rb1 induces apoptosis by altering the levels of P53, Bax, Cyto-c, Caspase-8, Caspase-3, Cleaved Caspase-3, Bcl-2, MMP-2, and MMP-9 proteins and activating the external apoptotic pathway.
Conclusion: Ginsenoside Rb1 inhibits proliferation and migration and induces apoptosis of 95D and NCI-H460 lung cancer cells by regulating the mitochondrial apoptotic pathway to achieve antitumor activity.
Keywords: Ginsenoside Rb1, lung cancer, 95D, NCI-H460, apoptosis, mechanism.
[http://dx.doi.org/10.1016/S0024-3205(00)00720-7] [PMID: 10972198]
[http://dx.doi.org/10.1016/j.apjtm.2016.04.007] [PMID: 27262071]
[http://dx.doi.org/10.1016/j.bbrc.2018.03.174] [PMID: 29596831]
[http://dx.doi.org/10.1016/j.rcl.2012.06.006] [PMID: 22974775]
[http://dx.doi.org/10.3892/mmr.2016.5753] [PMID: 27665734]
[http://dx.doi.org/10.4062/biomolther.2011.19.4.411]
[http://dx.doi.org/10.3892/or.2012.2136] [PMID: 23152132]
[http://dx.doi.org/10.3892/etm.2017.4889]
[http://dx.doi.org/10.1111/1440-1681.13708] [PMID: 36054718]
[http://dx.doi.org/10.3390/ijms221910458] [PMID: 34638797]
[http://dx.doi.org/10.1186/s12885-018-4299-4] [PMID: 29783929]
[http://dx.doi.org/10.1159/000503821] [PMID: 31671429]
[http://dx.doi.org/10.1007/s12272-021-01345-3] [PMID: 34302638]
[http://dx.doi.org/10.1007/s11626-023-00749-3] [PMID: 36790693]
[http://dx.doi.org/10.1371/journal.pone.0240533] [PMID: 33091036]
[http://dx.doi.org/10.3892/ijo.2017.4183] [PMID: 29115601]
[http://dx.doi.org/10.3892/mmr.2014.2821] [PMID: 25355274]
[http://dx.doi.org/10.12047/j.cjap.5891.2020.011] [PMID: 32476373]
[http://dx.doi.org/10.1016/j.jgr.2021.07.008] [PMID: 35058726]
[http://dx.doi.org/10.1186/s12906-019-2797-9] [PMID: 32020864]
[http://dx.doi.org/10.1016/j.biopha.2023.114870] [PMID: 37187019]
[http://dx.doi.org/10.3390/molecules26133926] [PMID: 34199025]
[http://dx.doi.org/10.3892/etm.2020.8543] [PMID: 32256776]
[http://dx.doi.org/10.1155/2022/1244836] [PMID: 35815288]
[http://dx.doi.org/10.18632/oncotarget.6451] [PMID: 26636541]
[http://dx.doi.org/10.1016/j.biopha.2019.109483] [PMID: 31629252]
[http://dx.doi.org/10.1016/j.cmet.2021.04.002] [PMID: 33915111]
[http://dx.doi.org/10.18632/oncotarget.16624] [PMID: 28402270]
[http://dx.doi.org/10.21873/anticanres.12082] [PMID: 29061814]
[http://dx.doi.org/10.1007/s13277-015-4078-7] [PMID: 26395261]
[http://dx.doi.org/10.1080/15384047.2020.1863120] [PMID: 33822672]
[http://dx.doi.org/10.1590/1678-7757-2022-0144] [PMID: 36074434]
[http://dx.doi.org/10.3892/ol.2021.12713] [PMID: 33907562]
[http://dx.doi.org/10.3390/ijms21249704] [PMID: 33352689]
[http://dx.doi.org/10.3892/ol.2018.9701] [PMID: 30655855]
[http://dx.doi.org/10.3892/ol.2019.10742] [PMID: 31579419]
[http://dx.doi.org/10.1155/2021/6815713] [PMID: 34603449]
[http://dx.doi.org/10.1039/C8FO01122B] [PMID: 30207362]
[http://dx.doi.org/10.1016/j.bcp.2019.07.008] [PMID: 31301277]
[http://dx.doi.org/10.1016/j.jgr.2016.04.003] [PMID: 28701864]
[http://dx.doi.org/10.1007/s12272-012-0416-0] [PMID: 22553065]
[http://dx.doi.org/10.3892/ijo.2015.3057] [PMID: 26094873]
[http://dx.doi.org/10.1007/s13238-014-0027-2] [PMID: 24622841]
[http://dx.doi.org/10.1016/j.ejphar.2011.06.008] [PMID: 21703260]
[http://dx.doi.org/10.2174/1871520623666230119092735] [PMID: 36655530]
[http://dx.doi.org/10.1080/21691401.2019.1643732] [PMID: 31313594]
[http://dx.doi.org/10.4103/pm.pm_583_21]
[http://dx.doi.org/10.1016/j.ejphar.2008.10.057] [PMID: 19022240]
[http://dx.doi.org/10.1007/BF03179933] [PMID: 12568360]
[http://dx.doi.org/10.1007/s12250-012-3220-6] [PMID: 22270803]
[http://dx.doi.org/10.1155/2022/3833489] [PMID: 35069931]
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