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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

H3K27ac激活的LncRNA NUTM2A-AS1通过MicroRNA-126-5p/FAM3C轴促进结直肠癌细胞的进展

卷 24, 期 12, 2024

发表于: 09 February, 2024

页: [1222 - 1234] 页: 13

弟呕挨: 10.2174/0115680096277956240119065938

价格: $65

Open Access Journals Promotions 2
摘要

目的：长链非编码RNA (Long non-coding RNA, lncRNAs)在结直肠癌(colorectal cancer, CRC)的发生发展过程中起着重要作用。然而，大多数lncRNA在结直肠癌中的功能和潜在的分子机制尚不清楚。 方法：采用定量实时聚合酶链反应(qRT-PCR)检测lncRNA NUTM2A-AS1在结直肠癌细胞系中的表达。采用细胞计数试剂盒8 (CCK-8)和流式细胞术检测lncRNA NUTM2A-AS1在结直肠癌细胞增殖和凋亡中的生物学功能。采用RT-qPCR和western blot检测细胞增殖相关蛋白、凋亡相关蛋白和FAM3C。利用生物信息学分析和双荧光素酶报告基因分析来确定ceRNA的相互调控机制。 结果：lncRNA NUTM2A-AS1在结直肠癌细胞系中显著升高，NUTM2A-AS1的沉默降低了细胞增殖，促进了细胞凋亡。在机制上，NUTM2A-AS1被在其启动子区域富集的赖氨酸27乙酰化组蛋白H3 (H3K27ac)转录激活，并且NUTM2A-AS1作为miR-126-5p的海绵，导致FAM3C在CRC细胞系中的表达上调。 结论：我们的研究提出NUTM2A-AS1作为一种致癌lncRNA，通过上调FAM3C表达促进结直肠癌恶性，这可能为结直肠癌的诊断和治疗提供新的见解和有希望的治疗靶点。

关键词: H3K27ac, lncRNA NUTM2A-AS1, miR-126-5p, FAM3C, ceRNA, CRC, 肿瘤生物标记物.

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