Title:Investigation of EpsA, OmpA, and Bap Genes among MDR and
XDR Acinetobacter baumannii Isolates in Khorramabad, Iran
Volume: 19
Issue: 4
Author(s): Iman Poladi, Pegah Shakib, Shahnaz Halimi, Somayeh Delfani, Farzad Ebrahim Zadeh and Faranak Rezaei*
Affiliation:
- Razi Herbal
Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
- Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Keywords:
Biofilm, multi-drug resistance, extensively drug-resistant, Acinetobacter baumannii, antibiotic resistance, clinical isolates.
Abstract:
Background: Acinetobacter baumannii is an opportunistic hospital pathogen
with high antibiotic resistance, and the ability to produce biofilm. This study aimed to investigate
epsA, ompA, and bap genes involved in biofilm formation in MDR and XDR
clinical isolates of Acinetobacter baumannii in Khorramabad, Iran.
Methods: In this study, 79 A. baumannii isolates were collected from various samples of
the patients admitted to tertiary hospitals in Khorramabad city, Iran, between January and
August 2019. After performing the semi-quantitative evaluation of biofilm production by
microtiter plate assay, screening of isolates carrying epsA, ompA, and bap genes was done
by PCR method. Finally, statistical analyses were conducted using SPSS 22.
Results: Among 79 A. baumannii isolates, 52% XDR, 40% MDR, and 16% non-XDRMDR
isolates were found to be biofilm producers. All XDR and 94% MDR isolates had
ompA and epsA genes, and bap genes were detected among > 80% of these isolates.
Moreover, the presence of biofilm-related genes and biofilm production among non-XDRMDR
isolates were less than among resistant isolates (p≤ 0.01).
Conclusion: Based on the results, biofilm production and simultaneous presence of epsA,
ompA, and bap genes among MDR, and XDR A. baumannii isolates have been found to be
significantly more than non-XDR-MDR isolates.
