Title:Evaluation of Antibacterial and Antibiofilm Activities of Fennel Seed Extract
Against Methicillin-resistant Staphylococcus aureus
Volume: 20
Issue: 2
Author(s): Ashish Sahu, Debaprasad Parai, Hari Ram Choudhary and Desh Deepak Singh*
Affiliation:
- Amity Institute of Biotechnology, Amity University, Rajasthan, Jaipur-303002, India
Keywords:
Fennel seed, MRSA, phytochemical, antibiotic resistance, biofilm, bactericidal activity.
Abstract:
Background: Antimicrobial resistance against pathogenic bacterial strains is the current
problem faced by the world. Medicinal plants are being focussed for new alternatives to routine
antibiotics for novel therapeutic and antimicrobial approaches.
Objective: In this present study, fennel extract from Foeniculum vulgare plant was investigated
against methicillin-resistant Staphylococcus aureus (MRSA).
Method: Ethanol extraction of fennel seeds was performed and minimum inhibitory concentration
(MIC), minimum bactericidal concentration (MBC), and time-kill curve was studied to find antimicrobial
activities. Biofilm eradication was estimated by crystal violet assay.
Result: Antimicrobial study showed a MIC of 500 μg/ml and MBC of 1000 μg/ml for fennel extract.
Sub-inhibitory concentrations (½ MIC and ¾ MIC) started to kill bacterial cells after the addition
of fennel extract at mid-log phase of growth. Test phytochemical eradicated 38.7%, 62.2%, and
76.9% of MRSA biofilm from microtitre plate when added at a concentration of 250 μg/ml, 375
μg/ml and 500 μg/ml, respectively.
Conclusion: This study showed different bactericidal and antibiofilm activity of fennel seed extract
against MRSA. We suggested that the phytocompound could be a promising candidate and further
investigation is needed to isolate the active component of the extract for the management of MRSA.