Title:The Production of Bioactive Peptides by Optimization of Enzymatic
Hydrolysis Process of Protein from Tilapia Fish Skin Waste (Oreochromis
niloticus, Linnaeus 1758) using Alcalase 2.4.L
Volume: 19
Issue: 10
Author(s): Siraj Salman Mohammad*, Maria Ivone M. J. Barbosa, Ormindo Gamallo and José L. Barbosa Junior
Affiliation:
- Departamento de Tecnologia de Alimentos, Universidade Federal Rural do Rio de Janeiro UFRRJ, CEP, Seropédica,
RJ, 23890-000, Brazil
Keywords:
Enzymes, bioactive peptides, antioxidants, amino acids, collagen, hydrolysates.
Abstract:
Aims: This study aimed at developing bioactive peptides by optimization of the enzymatic
hydrolysis process of protein from tilapia fish skin waste (Oreochromis niloticus, Linnaeus
1758) using alcalase 2.4.L.
Background: Natural bioactive peptides are considered to have low toxicity and therapeutic properties
as antioxidants.
Objective: The conditions of protein hydrolysis obtained from tilapia fish skin waste (Oreochromis
niloticus, Linnaeus 1758) were optimized using alcalase 2.4.l.
Methods: In this study, the hydrolysis of protein obtained from tilapia fish skin waste (TFSW) was
optimized using alcalase 2.4.L by central composite design (CCD). Degree of hydrolysis (DH), radical
scavenging activities (DPPH), and ferric-reducing antioxidant power (FRAP) were used as dependent
variables, whereas temperature, pH, and proportion of enzyme to the substrate (PE%) as
independent variables.
Results: The optimum degree of hydrolysis DH%, DPPH, and FRAP were achieved at a temperature
of 58.4℃, a pH of 8.7, except for DPPH, which was achieved at a pH of 7.0.
Conclusion: The present work demonstrated that TFSW could be used as a source to produce bioactive
peptides with significant antioxidant activities under specific conditions of enzymatic hydrolysis.