Simultaneous Determination of Six Bioactive Components of Astragali Radix in Rat Plasma Using Triple Quadrupole LC/MS and its Application to a Pharmacokinetic Study

Title:Simultaneous Determination of Six Bioactive Components of Astragali Radix in Rat Plasma Using Triple Quadrupole LC/MS and its Application to a Pharmacokinetic Study

Volume: 18 Issue: 10

Author(s): Yanlan Chai*, Juan Wang, Jing Wang, Kaishuo Zhang and Zi Liu*

Affiliation:

• Department of Radiotherapy Oncology, Tianjin Medical University Cancer Institute and Cancer Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, P.R. China

• Department of Radiotherapy Oncology, The First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China;

Keywords: Astragali Radix, triple quadrupole LC/MS, rat plasma pharmacokinetic, multiple reaction monitoring, acute radiation, Agilent Jet Steam System.

Abstract:

Background: To explore the pharmacokinetic data of Astragali Radix, a simple, one-step deproteinization procedure was followed to prepare plasma samples, and separation was achieved on an InfinityLab Poroshell 120 column (3.0 mm × 50 mm, 1.8 μm) with a gradient mobile phase consisting of solution A (water containing 0.1% formic acid) and solution B (methanol) at a flow rate of 0.3 mL/min.

Aims: In this study, a rapid, sensitive and selective Triple Quadrupole LC/MS method was developed to determine Calycosin-7-glucoside, Formononetin, Calycosin, Ononin, Cycloastragenoland Astragaloside IV from the extractive of Astragali Radixin in rat plasma, and it was validated for rat plasma as the matrix and applied for a pharmacokinetic study in rat plasma, while the internal standard was Sulfamethoxazole.

Objective: Multiple reaction monitoring (MRM) was used with an electrospray ionization source with the Agilent Jet Steam System (AJS-ESI) in the positive mode.

Methods: A rapid, sensitive and selective Triple Quadrupole LC/MS method was developed to determine Calycosin-7-glucoside, Formononetin, Calycosin, Ononin, Cycloastragenoland Astragaloside IV from the extractive of Astragali Radixin rat plasma, and it was validated for plasma as the matrix and applied for a pharmacokinetic study in rats, while the internal standard was Sulfamethoxazole.

Results: There were different pharmacokinetic characteristics after orally administering AR, including absorption and elimination rate, exposure degree, plasma distribution and retention rate.

Conclusion: Apart from that, a good linear response was observed within all analytes. The pharmacokinetic study on the six analytes in rats after oral administration of Astragali Radix was successfully completed by adopting this method, thus filling a blank in the pharmacokinetic studies of Astragali Radix.

Cite this article as:

Chai Yanlan*, Wang Juan, Wang Jing, Zhang Kaishuo and Liu Zi*, Simultaneous Determination of Six Bioactive Components of Astragali Radix in Rat Plasma Using Triple Quadrupole LC/MS and its Application to a Pharmacokinetic Study, Current Pharmaceutical Analysis 2022; 18 (10) . https://dx.doi.org/10.2174/1573412918666220922120815