Title:In vitro α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia alba Blume
Volume: 23
Issue: 9
Author(s): Swagat K. Das*, Sagarika Dash, Hrudayanath Thatoi and Jayanta K. Patra*
Affiliation:
- Department of Biotechnology, College of Engineering and Technology, Techno Campus, Ghatikia, Bhubaneswar 751003, Odisha,India
- Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi 10326,Korea
Keywords:
Avicennia alba, antidiabetic, antioxidant, anti-inflammatory, GC-MS analysis, cytotoxic.
Abstract:
Background: Avicennia alba Blume, is a well-known mangrove plant used in traditional
medicinal practices for several human ailments.
Objective: The study aimed at the evaluation of antidiabetic, antioxidant, anti-inflammatory and
cytotoxic activities of A. alba ethanolic leaf (AAL) and bark (AAB) extract along with
phytochemical investigation.
Methods: In vitro antidiabetic study was done by α-amylase, α-glucosidase enzyme inhibition
assay; antioxidant study by DPPH, ABTS, superoxide, and metal chelating assays, antiinflammatory
study by protein denaturation assay. The cytotoxicity study was done on TC1 murine
cell line. Further, GC-MS analysis was carried out for AAL extracts.
Results: AAL exhibited better antidiabetic activities with IC50 values of 1.18 and 0.87 mg/ml
against α-amylase and α-glucosidase enzymes respectively. The AAL exhibited better ABTS,
superoxide scavenging and metal chelating potential with IC50 values of 0.095, 0.127 and 0.444
mg/ml. However, AAB showed higher DPPH scavenging potential with IC50 value of 0.163
mg/ml. The AAL also exhibited higher protein denaturation potential with IC50 value of 0.370
mg/ml. The bark extract exhibited better cytotoxic activity as compared to leaf extracts on the TC1
murine cell line. The phytochemical study revealed higher total phenol (25.64 mg GAE/g),
flavonoid (205.09 mg QE/g), and tannin content (251.17 mg GAE/g) in AAL. The GC-MS analysis
revealed the presence of several compounds in AAL extract.
Conclusion: The result of the present study highlights the antidiabetic, antioxidant and cytotoxic
activities of mangrove plant Avicennia alba.