Differential Effects of Antofine N-Oxide on Solid Tumor and Leukemia Cells

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)

Volume 16, 12 Issues, 2016

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Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

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  • 27th of 59 in Chemistry, Medicinal

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Michelle Prudhomme
Universite Blaise Pascal - C.N.R.S
Aubiere Cedex

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Differential Effects of Antofine N-Oxide on Solid Tumor and Leukemia Cells

Anti-Cancer Agents in Medicinal Chemistry, 14(10): 1315-1323.

Author(s): Tania Bour, Xianwen Yang, Weihong Li, Francois Bernardin, Tony Kaoma, Arnaud Muller, Laurent Vallar and Andre Steinmetz.

Affiliation: Laboratory of Cellular and Molecular Oncology, Public Research Center for Health (CRP-Sante), L-1526 Luxembourg.


We have studied the anti-cancer activities of antofine N-oxide isolated and purified from the medicinal plant Cynanchum vincetoxicum. Antofine N-oxide displayed a strong inhibitory effect on several solid tumor cell lines (glioblastoma, breast carcinoma and lung carcinoma) and on a T-cell leukemia cell line. Remarkably, its cytotoxic effect was considerably weaker in non-cancer cells. Antofine N-oxide was found to inhibit proliferation of the solid tumor cells whereas it caused apoptotic cell death in the leukemia cells. A microarray analysis after a short treatment revealed that the number of differentially expressed genes was considerably higher in solid tumor than in leukemia cells. Up-regulated genes in the three solid tumor cell lines include genes related to TNFα signaling, of which TNFα was among the most significantly induced. A functional analysis revealed that TNFR1 signaling was most likely activated in the solid tumor cells. The increased mRNA levels of several genes of this pathway (namely TNFα, TNFAIP3 and BIRC3) were confirmed by real-time quantitative PCR after different treatment durations. Finally a slight inhibition of NFκB-mediated transcription was observed in the same cells. Together our results suggest that inhibition of cell proliferation in solid tumor cells essentially occurs through TNFα signaling whereas this pathway is not activated in leukemia cells. Apoptotic cell death in the latter is induced by a distinct yet unknown pathway.


Apoptosis, cytostasis, leukemia, microarrays, phenanthroindolizidine alkaloid, solid tumor, TNFα.

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Article Details

Volume: 14
Issue Number: 10
First Page: 1315
Last Page: 1323
Page Count: 9
DOI: 10.2174/1871520614666140624110458
Price: $58

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