Triplex-Forming Oligonucleotides as Potential Tools for Modulation of Gene Expression

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)


Volume 16, 12 Issues, 2016


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Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

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  • 27th of 59 in Chemistry, Medicinal

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Editor-in-Chief:
Michelle Prudhomme
Universite Blaise Pascal - C.N.R.S
Aubiere Cedex
France


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Triplex-Forming Oligonucleotides as Potential Tools for Modulation of Gene Expression



Anti-Cancer Agents in Medicinal Chemistry, 5(4): 319-326.

Author(s): F. A. Rogers, J. A. Lloyd and P M Glazer.

Affiliation: Department of TherapeuticRadiology and Genetics, Yale University School of Medicine, 15 York St.,HRT 313, New Haven, CT 06520, USA.

Abstract

Triplex-forming oligonucleotides (TFOs) bind in the major groove of duplex DNA at polypurine/ polypyrimidine stretches in a sequence-specific manner. The binding specificity of TFOs makes them potential candidates for use in directed genome modification. A number of studies have shown that TFOs can introduce permanent changes in a target sequence by stimulating a cells inherent repair pathways. TFOs have also been demonstrated to inhibit gene expression providing a possible role for these compounds in cancer therapy. This review summarizes the dual roles of TFOs for use in delivering DNA reactive compounds to a specific site in the genome or for introducing permanent changes in the target sequence through the introduction of an altered helical structure. In addition to compiling the ways in which TFOs have been successfully utilized, this review will explore conflicting reports of TFO bioactivity focusing on the variables which affect the efficacy in vitro of TFO mediated genomic modification which in turn may represent the obstacles encountered using TFOs to modulate gene expression in vivo.

Keywords:

triplex-forming oligonucleotides, gene targeting, anti-cancer, repair, recombination, review.



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Article Details

Volume: 5
Issue Number: 4
First Page: 319
Last Page: 326
Page Count: 8
DOI: 10.2174/1568011054222300
Price: $58
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