Recent Advances in Protein Tyrosine Phosphatase Detection Using Chemical Probes

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)

Volume 16, 12 Issues, 2016

Download PDF Flyer

Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

Aims & ScopeAbstracted/Indexed in

Ranking and Category:
  • 27th of 59 in Chemistry, Medicinal

Submit Abstracts Online Submit Manuscripts Online

Michelle Prudhomme
Universite Blaise Pascal - C.N.R.S
Aubiere Cedex

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 2.722
5 - Year: 2.849

Recent Advances in Protein Tyrosine Phosphatase Detection Using Chemical Probes

Anti-Cancer Agents in Medicinal Chemistry, 11(1): 54-63.

Author(s): Kyeong Lee, Hyo Jin Kang, Yan Xia and Sang J Chung.

Affiliation: BioNanotechnology Research Center, KRIBB and Nanobio Major, UST, Yuseong, Daejeon 305-333, Korea.


Protein tyrosine phosphatases (PTPs) are key regulatory enzymes in signal transduction pathways and their aberrancy has been implicated in various diseases such as cancers, metabolic syndromes, and autoimmune disorders. In spite of its great importance, determination of the functional significance of PTPs remains a major challenge, and efficient methodologies are needed to specifically delineate PTP functions. Besides the strategy to use potent and selective PTP inhibitors to study the physiological roles of the enzymes, measurement of PTP activities using specific PTP substrates or activity-based probes (ABPs) has been reported. This review focused on the recent development of small molecular probes to detect PTP activities, consisting of five sub-categories: 1. Conventional fluorescent substrates; 2. Ratiometric fluorescent PTP substrates; 3. Fluorescence substrates with selectivity to a single PTP or a class of PTPs; 4. ABPs specific for PTPs; and 5. A real-time imaging of PTP-substrate complex using a small molecule PTP probe which, offers a measurement of a real-time activity of a certain PTP in cells.


DiFMUP, in-gel assay, phosphotyrosine, dual-specificity PTP (DUSP), Forster resonance energy transfer (FRET), 4-fluoromethylphenylphosphate (FMPP), 2-fluoromethylphosphotyrosine (FMPT), PTKs, PTPs, Pnpp.

Download Free Order Reprints Order Eprints Rights and Permissions

Article Details

Volume: 11
Issue Number: 1
First Page: 54
Last Page: 63
Page Count: 10
DOI: 10.2174/187152011794941208

Related Journals

Webmaster Contact: Copyright © 2016 Bentham Science