Berberine Exhibits Antitumor Effects in Human Ovarian Cancer Cells

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)

Volume 16, 12 Issues, 2016

Download PDF Flyer

Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

Aims & ScopeAbstracted/Indexed in

Ranking and Category:
  • 27th of 59 in Chemistry, Medicinal

Submit Abstracts Online Submit Manuscripts Online

Michelle Prudhomme
Universite Blaise Pascal - C.N.R.S
Aubiere Cedex

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 2.722
5 - Year: 2.849

Berberine Exhibits Antitumor Effects in Human Ovarian Cancer Cells

Anti-Cancer Agents in Medicinal Chemistry, 15(4): 511-516.

Author(s): Ping Jin, Chunjie Zhang and Na Li.

Affiliation: Shenzhen Maternity & Child Healthcare Hospital Affiliated to Southern Medical University China.


Background: Berberine is an extract of a traditional Chinese herbal medicine and has been shown to inhibit the proliferation and induce apoptosis in a wide variety of tumour cells. However, the effects of Berberine in ovarian cancer cells are unknown.

Aims: To investigate the potential anti-cancer effects of Berberine in human ovarian cancer cells.

Methods: Cell proliferation was evaluated by a MTT assay. Apoptosis was determined by using Annexin V/PI staining and transmission electron microscopy. The methylation status of the hMLH1 promoter CpG islands was analysed by using methylation-specific polymerase chain reaction (MSP). mRNA expression of BCLM-2, BAX, survivin and HMLH1 was quantified by real-time fluorescence quantitative RT-PCR.

Results: Berberine significantly inhibited the proliferation of SKOV3 cells in a dose- and time-dependent manner. It also dosedependently induced apoptosis, possibly through down-regulating the anti-apoptotic genes BCL-2 and survivin, and up-regulating the pro-apoptotic gene BAX. When combined with cisplatin, Berberine showed a strong synergistic anticancer effect against ovarian cancer cells. In addition, Berberine was found to restore the demethylation status of the hMLH1 promoter and up-regulate the mRNA expression of hMLH1.

Conclusion: Berberine possesses antitumor effect via inhibition of cell proliferation and induction of apoptosis in ovarian cancer cells. Berberine could synergistically enhance the cell killing effect of other antitumor agents such as cisplatin. Further studies are essential to explore the therapeutic potential of Berberine either alone or in combination with other anticancer agents in patients with ovarian cancer.


Berberine, methylation, mismatch repair genes, ovarian cancer, SKOV3 cells.

Purchase Online Order Reprints Order Eprints Rights and Permissions

Article Details

Volume: 15
Issue Number: 4
First Page: 511
Last Page: 516
Page Count: 6
DOI: 10.2174/1871520614666141226124110
Price: $58

Related Journals

Webmaster Contact: Copyright © 2016 Bentham Science