Intracellular Distribution and Biological Effects of Phytochemicals in a Sex Steroid- Sensitive Model of Human Prostate Adenocarcinoma

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)

Volume 17, 14 Issues, 2017

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Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

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Michelle Prudhomme
Institut de Chimie de Clermont-Ferrand
Université Clermont Auvergne

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Intracellular Distribution and Biological Effects of Phytochemicals in a Sex Steroid- Sensitive Model of Human Prostate Adenocarcinoma

Anti-Cancer Agents in Medicinal Chemistry, 14(10): 1386-1396.

Author(s): Antonella Smeriglio, Domenico Trombetta, Daniele Marcoccia, Laura Narciso, Alberto Mantovani and Stefano Lorenzetti.

Affiliation: ISS - Istituto Superiore di Sanità, Dpt. of Food Safety and Veterinary Public Health, Food and Veterinary Toxicology Unit, viale Regina Elena, 299 - 00161 Rome, Italy.


Prostate function is critical for male fertility and its well-known oncological biomarker, namely Prostate-Specific Antigen (PSA), can be also used to monitor prostate epithelial human cells upon treatment with pharmaceutical drugs or natural bioactive compounds.

The LNCaP human prostate cell line was previously set up as a model system to investigate chemicals affecting prostate epithelium functionality by means of a tiered approach integrating two different toxicological endpoints, cell viability (MTS) and PSA secretion assays. Here, the same approach has been used to characterize the biological effects of phytochemicals on prostate epithelium. The antiandrogenic ability of phytochemicals to inhibit DHT-induced PSA secretion has been investigated also characterizing their intracellular distribution, in the presence or absence of sex steroids. Intracellular distribution allows to verify whether and to which extent each phytochemical is able to enter the cell and to reach the nucleus, the latter being the target of the supposed transcriptional modulatory activity upon phytochemicals’ binding to sex steroid receptors.

Some phytochemicals, supposed to have a role in the functionality of the prostate epithelium, have been tested in a dose-dependent manner in both MTS and PSA secretion assays. In parallel, to establish the “effective concentration”, in comparison to the “nominal one”, the intracellular amount of each phytochemical has been assessed upon cell fractionation of LNCaP-treated cells and subsequent chromatographic measurements.


Androgen receptor, biomarker, cytotoxicity, flavonoids, intracellular distribution, nominal vs effective concentration, PSA secretion, testing strategy.

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Article Details

Volume: 14
Issue Number: 10
First Page: 1386
Last Page: 1396
Page Count: 11
DOI: 10.2174/1871520614666140624111011
Price: $58

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