Selection and Characterization of Human Anti-MAGE-A1 scFv and Immunotoxin

ISSN: 1875-5992 (Online)
ISSN: 1871-5206 (Print)

Volume 16, 12 Issues, 2016

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Anti-Cancer Agents in Medicinal Chemistry

Formerly: Current Medicinal Chemistry - Anti-Cancer Agents

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  • 27th of 59 in Chemistry, Medicinal

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Michelle Prudhomme
Universite Blaise Pascal - C.N.R.S
Aubiere Cedex

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Selection and Characterization of Human Anti-MAGE-A1 scFv and Immunotoxin

Anti-Cancer Agents in Medicinal Chemistry, 13(8): 1259-1266.

Author(s): Hong Lin, Yuan Mao, Da-Wei Zhang, Hu Li, Jin-Rong Qiu, Jin Zhu and Ren-Jie Chen.

Affiliation: Department of Otolaryngology-Head and Neck Surgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China.


Melanoma-associated antigen (MAGE) is expressed on the surface of multiple tumor cell types and is a promising target of biotherapeutic drug delivery via the anti-MAGE-A1 antibody. In this study, a human single-chain variable fragment (scFv) antibody phage library was generated and applied to recombinant MAGE-A1-coated immunotubes by phage display technology. The soluble anti- MAGE-A1 scFv was expressed and purified by immobilized metal-chelated affinity chromatography (IMAC). The anti-MAGE-A1 scFv could bind native MAGE-A1 confirmed by enzyme-linked immunosorbent assay (ELISA), dot blot, and immunoprecipitation (IP) analysis. The immunotoxin was expressed and purified by IMAC successfully. The results indicated that the human anti-MAGE-A1 immunotoxin could provide a valuable drug for clinic cancer therapy.


Phage display antibody library, anti-MAGE-A1 scFv fragment, immunotoxin.

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Article Details

Volume: 13
Issue Number: 8
First Page: 1259
Last Page: 1266
Page Count: 8
DOI: 10.2174/18715206113139990134
Price: $58

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